Rat Hemoglobin ELISA Kit | RB1909 elisa kit
Rat Hemoglobin ELISA Kit
Reactivity
Rat
Synonyms
Hemoglobin; Rat Hemoglobin ELISA Kit; Rat Hemoglobin; RB1909 elisa kit
Reactivity
Rat
Samples
Immunoperoxidase Assay for Determination of Hemoglobin in Rat Samples
Assay Type
Sandwich
Intended Uses
The Hemoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemoglobin in biological samples of rats.
Preparation and Storage
Store at 4 degree C.
Related Product Information for RB1909 elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HM in the test sample. The quantity of HM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Hemoglobin (HM) is the iron-containing oxygen transport protein in the red blood cells. It picks up oxygen in the lungs and delivers it to the tissues to maintain the viability of cells. Each subunit of hemoglobin is a globular protein with an embedded heme group. Each heme group contains an iron atom and is responsible for the binding of oxygen. Decrease of hemoglobin leads to symptoms of anemia.
Background: Hemoglobin (HM) is the iron-containing oxygen transport protein in the red blood cells. It picks up oxygen in the lungs and delivers it to the tissues to maintain the viability of cells. Each subunit of hemoglobin is a globular protein with an embedded heme group. Each heme group contains an iron atom and is responsible for the binding of oxygen. Decrease of hemoglobin leads to symptoms of anemia.
Product Categories/Family for RB1909 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hemoglobin
NCBI Official Symbol
RB1909
NCBI Protein Information
hemoglobin
Protein Family
Similar Products
Product Notes
The Rat RB1909 (Catalog #AAA564115) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564115 ELISA Kit recognizes Rat RB1909. It is sometimes possible for the material contained within the vial of "Hemoglobin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
