Porcine Coagulation factor VII (F7) ELISA Kit | F7 elisa kit
Pig Coagulation factor VII (F7) ELISA Kit
Gene Names
F12; FXII
Reactivity
Porcine
Synonyms
Coagulation factor VII (F7); Pig Coagulation factor VII (F7) ELISA Kit; HAE3; HAEX; HAF; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XII; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; F7 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig cTn-T. No significant cross-reactivity or interference between Pig cTn-T and analogues was observed.
Sequence Length
616
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.52 ng/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for F7 elisa kit
Intended Uses: For the quantitative detection of Pig Cardiac Troponin T (cTn-T) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate cTn-T in Pig serum, plasma. An antibody specific for cTn-T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-T bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate cTn-T in Pig serum, plasma. An antibody specific for cTn-T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTn-T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-T bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,012 Da
NCBI Official Full Name
coagulation factor XII
NCBI Official Symbol
F12
NCBI Official Synonym Symbols
FXII
NCBI Protein Information
coagulation factor XII
UniProt Protein Name
Coagulation factor XII
Protein Family
UniProt Gene Name
F12
UniProt Synonym Gene Names
HAF
Uniprot Description
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa ().
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Product Notes
The Porcine F7 f12 (Catalog #AAA2800137) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2800137 ELISA Kit recognizes Porcine F7. It is sometimes possible for the material contained within the vial of "Coagulation factor VII (F7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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