Sheep TYROSINE HYDROXYLASE Polyclonal Antibody | anti-Th antibody

SHEEP ANTI RAT TYROSINE HYDROXYLASE

Cat. Num. AAA223866

• Gene Names: Th; The
• Reactivity: Mammals (Expected from Sequence), Rabbit
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Synonyms: TYROSINE HYDROXYLASE; Polyclonal Antibody; SHEEP ANTI RAT TYROSINE HYDROXYLASE; anti-Th antibody

• Host: Sheep
• Reactivity: Mammals (Expected from Sequence), Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for Tyrosine hydroxylase (TH). Tyrosine hydroxylase catalyses the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. ; Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 498

• Applicable Applications for anti-Th antibody: Immunohistology Frozen, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 60kDa in rat caudate cell lysates.; Immunohistology - Frozen: Maximum Dilution: 1/1000; Immunofluorescence: Maximum Dilution: 1/1000; Western Blotting: Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Rat Tyrosine hydroxylase purified from pheochromocytoma.; Buffer Solution
• Antiserum Preparation: Antisera to tyrosine hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by immunoaffinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-Th antibody

MBS223866 is specific for Tyrosine hydroxylase (TH). Tyrosine hydroxylase catalyses the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.

NCBI and Uniprot Product Information

• NCBI GI #: 6981652
• NCBI GeneID: 25085
• NCBI Accession #: NP_036872.1
• NCBI GenBank Nucleotide #: NM_012740.3
• UniProt Accession #: P04177
• Molecular Weight: 55,966 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: Th
• NCBI Official Synonym Symbols: The
• NCBI Protein Information: tyrosine 3-monooxygenase; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: Th
• UniProt Synonym Gene Names: TH
• UniProt Entry Name: TY3H_RAT

NCBI Description

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Vesicle; EC 1.14.16.2; Endoplasmic reticulum; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - tyrosine

Cellular Component: neuron projection; internal side of plasma membrane; cytoplasmic vesicle membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cell soma; axon; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; monooxygenase activity; tyrosine 3-monooxygenase activity

Biological Process: response to nicotine; heart development; response to lipopolysaccharide; dopamine biosynthetic process; response to organic cyclic substance; phytoalexin metabolic process; protein homotetramerization; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to drug; response to light stimulus; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to steroid hormone stimulus; cerebral cortex development; response to activity; response to water deprivation; synaptic transmission, dopaminergic; response to metal ion; response to peptide hormone stimulus; locomotory behavior; response to insecticide; fatty acid metabolic process; response to salt stress; response to estradiol stimulus; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; memory; synaptic vesicle amine transport; response to hypoxia; embryonic camera-type eye morphogenesis; cognition