Rabbit anti-Human TRPM2 Polyclonal Antibody | anti-TRPM2 antibody

TRPM2 Rabbit pAb

Cat. Num. AAA8545925

• Gene Names: TRPM2; KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; LTrpC-2; NUDT9L1
• Reactivity: Human
• Applications: Immunofluorescence
• Purity: Affinity Purified
• Synonyms: TRPM2; Polyclonal Antibody; TRPM2 Rabbit pAb; KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; LTrpC-2; NUDT9L1; anti-TRPM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
• Sequence Length: 1503

• Applicable Applications for anti-TRPM2 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:100
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1204-1503 of human TRPM2 (NP_003298.1).
• Cellular Localization: Cell Membrane, Cell Projection, Cytoplasmic Vesicle, Lysosome, Membrane
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-TRPM2 antibody

Isoform 1: Nonselective, voltage-independent cation channel that mediates Na+ and Ca2+ influx, leading to increased cytoplasmic Ca2+ levels (PubMed:11960981, PubMed:12594222, PubMed:11385575, PubMed:11509734, PubMed:11804595, PubMed:15561722, PubMed:16601673, PubMed:19171771, PubMed:20660597, PubMed:27383051, PubMed:27068538). Extracellular calcium passes through the channel and increases channel activity by binding to the cytoplasmic domain and stabilizing the channel in an open conformation (PubMed:19171771). Also contributes to Ca2+ release from intracellular stores in response to ADP-ribose (PubMed:19454650). Plays a role in numerous processes that involve signaling via intracellular Ca2+ levels (Probable). Besides, mediates the release of lysosomal Zn2+ stores in response to reactive oxygen species, leading to increased cytosolic Zn2+ levels (PubMed:25562606, PubMed:27068538). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Activated by intracellular ADP-ribose, beta-NAD (NAD+) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:11960981, PubMed:11385575, PubMed:11509734, PubMed:11804595, PubMed:15561722, PubMed:16601673, PubMed:19171771, PubMed:27383051, PubMed:27068538). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate (PubMed:20650899, PubMed:25918360). Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius) (PubMed:22493272). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca2+ levels. Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca2+ levels. Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca2+ and Zn2+ levels (PubMed:27068538). Confers susceptibility to cell death following oxidative stress (PubMed:12594222, PubMed:25562606).

NCBI and Uniprot Product Information

• NCBI GI #: 160381681
• NCBI GeneID: 7226
• UniProt Accession #: O94759
• Molecular Weight: 95kDa; 165kDa; 171kDa
• NCBI Official Full Name: Transient receptor potential cation channel subfamily M member 2
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 2
• NCBI Official Symbol: TRPM2
• NCBI Official Synonym Symbols: KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; LTrpC-2; NUDT9L1
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 2
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 2
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM2
• UniProt Synonym Gene Names: EREG1; KNP3; LTRPC2; TRPC7; LTrpC-2; LTrpC2; TrpC7
• UniProt Entry Name: TRPM2_HUMAN

NCBI Description

The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2016]

Uniprot Description

TRPM2: Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; EC 3.6.1.13; Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: ADP-ribose diphosphatase activity; calcium channel activity; manganese ion transmembrane transporter activity; sodium channel activity

Biological Process: response to hydroperoxide; metabolic process; calcium ion transport; transmembrane transport