Rabbit anti-Human, Mouse PUMA, alpha Polyclonal Antibody | anti-BBC3 antibody

PUMA, alpha (p53 Upregulated Modulator of Apoptosis)

Cat. Num. AAA618696

• Gene Names: BBC3; JFY1; PUMA; FLJ42994
• Reactivity: Human, Mouse
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PUMA; alpha; Polyclonal Antibody; alpha (p53 Upregulated Modulator of Apoptosis); Anti -PUMA; anti-BBC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human control peptide sequence is not found in alternatively spliced PUMA-beta, gamma or delta. 100% conserved in mouse PUMA-alpha. No significant sequence homology is detected with other proteins.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml for affinity pure.
• Immunogen: Synthetic peptide 19-aa near the N-terminus of Human PUMA
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-BBC3 antibody

p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. P53 tumor-suppressor protein functions to inhibit the growth of tumor cells. Although p53 is not required for the growth and differentiation of normal cells, it may play a role in several cellular processes including senescence and differentiation. Mice lacking p53 show a high incidence of tumor formation. P53 inhibits cell growth through inactivation of both cell cycle arrest and apoptosis. Several p53-induces genes play a role in the induction of apoptosis. Through global profiling of genes that were expressed soon after p53 expression, a novel gene termed PUMA (p53-upregulated modulator of apoptosis) as a target for p53 activation. PUMA/JFY1 (mouse 193-aa, human 193-aa, chromosome 19q) is expressed in most tissues. It is exclusively located in mitochondria and it induces cytochrome-C release. PUMA binds to BCL2 and BCLXL through a BH3 domain (141-149 aa). Exogenous expression of PUMA resulted in an extremely rapid and profound apoptosis that occurred much earlier than that resulting from exogenous expression of p53. PUMA is alternatively spliced to produce 2-4 transcripts PUMA alpha (193-aa, ~25kD), -beta (131-aa, ~16kD), gamma, and delta (101-aa). The gamma and delta forms were not detectable in endogenous cells. PUMA alpha and beta displayed similar apoptosis inducing ability.

Product Categories/Family for anti-BBC3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 13785988
• NCBI GeneID: 27113
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 20,532 Da
• NCBI Official Full Name: PUMA alpha
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; FLJ42994
• NCBI Protein Information: bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth