Rabbit IKBKAP Polyclonal Antibody | anti-IKBKAP antibody

IKBKAP Antibody

Cat. Num. AAA9629897

• Gene Names: IKBKAP; FD; DYS; ELP1; IKAP; IKI3; TOT1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: IKBKAP; Polyclonal Antibody; IKBKAP Antibody; DKFZp781H1425; DYS; Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III); Elongator complex protein 1; ELP 1; ELP1; ELP1_HUMAN; FD; FLJ12497; IKAP; IkappaB kinase complex associated protein; IkappaB kinase complex-associated protein; ikbkap; IKI 3; IKI3; IKK complex associated protein; IKK complex-associated protein; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein; OTTHUMP00000063889; p150; TOT 1; TOT1; anti-IKBKAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: IKBKAP Antibody detects endogenous levels of IKBKAP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IKBKAP antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human IKBKAP.
• Conjugation: Unconjugated
• Similarity: Belongs to the ELP1/IKA1 family.
• Subunit Structure: Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta.
• Function: Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Involved in neurogenesis (By similarity). Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (By similarity). May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-IKBKAP antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 3757822
• NCBI GeneID: 8518
• UniProt Accession #: O95163; Q5JSV2; Q9H327; Q9UG87
• Molecular Weight: 150,254 Da
• NCBI Official Full Name: IkappaB kinase complex associated protein
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
• NCBI Official Symbol: IKBKAP
• NCBI Official Synonym Symbols: FD; DYS; ELP1; IKAP; IKI3; TOT1
• NCBI Protein Information: elongator complex protein 1; p150; IKK complex-associated protein; ikappaB kinase complex-associated protein; elongator acetyltransferase complex subunit 1
• UniProt Protein Name: Elongator complex protein 1
• UniProt Gene Name: IKBKAP
• UniProt Synonym Gene Names: ELP1; IKAP; ELP1; IKK complex-associated protein
• UniProt Entry Name: ELP1_HUMAN

NCBI Description

The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq, Jul 2008]

Uniprot Description

IKBKAP: May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK). Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta. Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Belongs to the ELP1/IKA1 family.

Protein type: Protein kinase, regulatory subunit; Transcription regulation

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex

Molecular Function: protein binding; signal transducer activity; phosphorylase kinase regulator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; immune response; protein complex assembly; signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Iii