Rabbit GALNT1 Polyclonal Antibody | anti-GALNT1 antibody

GALNT1 Antibody

Cat. Num. AAA9630305

• Gene Names: GALNT1; GALNAC-T1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GALNT1; Polyclonal Antibody; GALNT1 Antibody; GALNAC T1; GalNAc transferase 1; GalNAc-T1; GALNT 1; GALT1_HUMAN; Polypeptide GalNAc transferase 1; Polypeptide N acetylgalactosaminyltransferase 1; Polypeptide N-acetylgalactosaminyltransferase 1 soluble form; pp GaNTase 1; pp-GaNTase 1; Protein UDP acetylgalactosaminyltransferase 1; Protein-UDP acetylgalactosaminyltransferase 1; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1; UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1); UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1; anti-GALNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GALNT1 Antibody detects endogenous levels of GALNT1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GALNT1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GALNT1.
• Conjugation: Unconjugated
• Similarity: There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding. The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates (By similarity). Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
• Tissue Specificity: Widely expressed. Expressed in all tissues tested.
• Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GALNT1 antibody

Golgi apparatus; Golgi stack membrane; Single-pass type II membrane protein; Secreted; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 13124891
• NCBI GeneID: 2589
• NCBI Accession #: NP_065207.2
• NCBI GenBank Nucleotide #: NP_065207.2
• UniProt Accession #: Q10472
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 1
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 1
• NCBI Official Symbol: GALNT1
• NCBI Official Synonym Symbols: GALNAC-T1
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 1
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 1
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT1
• UniProt Synonym Gene Names: GalNAc-T1; pp-GaNTase 1

NCBI Description

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.