Rabbit anti-Rat EP3 Receptor Polyclonal Antibody
EP3 Receptor
Reactivity
Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
EP3 Receptor; Polyclonal Antibody; Anti -EP3 Receptor; anti-EP3 Receptor antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the rat EP3 Receptor.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-EP3 Receptor antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Dilution: ELISA: 0.5-1ug/ml using 1ug/ml of control peptide E3364-30
Western Blot: 1-10ug/ml using ECL. EP3 is ~62kD protein in the kidney.
Immunohistochemistry: 2-20ug/ml. Reported to work with mouse and human retina and human gastric tissues (7, 8).
Western Blot: 1-10ug/ml using ECL. EP3 is ~62kD protein in the kidney.
Immunohistochemistry: 2-20ug/ml. Reported to work with mouse and human retina and human gastric tissues (7, 8).
Immunogen
Synthetic peptide corresponding to 15aa from the N-terminal, extracellular domain of the rat EP3 Receptor (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-EP3 Receptor antibody
Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3 and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus. EP3 is expressed in the kidney, brain, stomach, uterus, liver, skeletal muscle, intestine and thymus. Alternative splicing of EP3 produces various isoforms with different C-terminal tails. EP3 isoforms have identical ligand binding but different coupling properties with G-proteins: alpha and beta coupled to Gi, whereas gamma couples with Gi and Gs. EP3 knockout mice show impaired febrile response to pyrogens.
Product Categories/Family for anti-EP3 Receptor antibody
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Product Notes
The EP3 Receptor (Catalog #AAA614907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EP3 Receptor reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EP3 Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Dilution: ELISA: 0.5-1ug/ml using 1ug/ml of control peptide E3364-30 Western Blot: 1-10ug/ml using ECL. EP3 is ~62kD protein in the kidney. Immunohistochemistry: 2-20ug/ml. Reported to work with mouse and human retina and human gastric tissues (7, 8). Researchers should empirically determine the suitability of the EP3 Receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EP3 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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