Rabbit Clathrin Light Chain (CLCa/CLCb) Polyclonal Antibody | anti-CLCa/CLCb antibody
Clathrin Light Chain (CLCa/CLCb) Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Synonyms
Clathrin Light Chain (CLCa/CLCb); Polyclonal Antibody; Clathrin Light Chain (CLCa/CLCb) Rabbit Polyclonal Antibody; Clathrin light chain A; Lea; anti-CLCa/CLCb antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody recognizes the N-terminal portion of Clathrin Light Chain
Form/Format
Lyophilized immunogen affinity purified in PBS pH7.4
Applicable Applications for anti-CLCa/CLCb antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
If reconstituted in 200 muL: IF: 1:100 to 1:500; WB: 1:1000-1:2000
Immunogen
Peptide in N term
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CLCa/CLCb antibody
Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.
References
1. Allaire PD-Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis. 2. Ybe JA-Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. 3. Girard M-Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles. 4. Hayashi M-Cell-and stimulus-dependent heterogeneity of synaptic vesicle endocytic recycling mechanisms revealed by studies of dynamin 1-null neurons. 5. Poupon V-Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.
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Product Notes
The CLCa/CLCb (Catalog #AAA588253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Clathrin Light Chain (CLCa/CLCb) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Clathrin Light Chain (CLCa/CLCb) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). If reconstituted in 200 muL: IF: 1:100 to 1:500; WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the CLCa/CLCb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clathrin Light Chain (CLCa/CLCb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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