Rabbit anti-Mouse Cadherin 14, 15, M Polyclonal Antibody | anti-CDH15 antibody

Cadherin 14, 15, M

Cat. Num. AAA630309

• Gene Names: CDH15; CDH3; CDHM; MCAD; MRD3; CDH14
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cadherin 14; 15; M; Polyclonal Antibody; Anti -Cadherin 14; anti-CDH15 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Speci c for the mouse M-Cadherin protein. On Western blots, it identi es a single band at ~130kD. Reactivity has been con rmed with fetal mouse muscle homogenates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-CDH15 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blotting.; Dilution: ELISA: 0.1-1ug/mL; Western Blotting: 1-3ug/mL
• Immunogen: Synthetic peptide derived from the C terminal region of the mouse M-Cadherin (Cadherin 14, Cadherin 15) protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CDH15 antibody

M-cadherin belongs to the cadherin family of proteins, which are calcium-dependent, protease-sensitive molecules that mediate homotypic cell-cell adhesion. M-cadherin is highly expressed in developing and regenerating skeletal muscles.1 In postnatal mature myofibers and after completion of myotube formation in vitro, M-cadherin expression is downregulated, but the protein remains present in quiescent satellite cells.2 M-cadherin is also present in the contactus adherens in the granular cell layer of the cerebellar glomerulus.3 Studies of skeletal muscle and cerebellum in M-cadherin-null mutant mice have demonstrated that other cadherins such as N-cadherin and R-cadherin may compensate for absent M-cadherin function.4 Although the calculated molecular weight of M-cadherin is ~86kDa, the apparent molecular molecular weight is ~130kDa due to glycosylation.

Product Categories/Family for anti-CDH15 antibody

Antibodies; Abs to Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 4826669
• NCBI GeneID: 1013
• NCBI Accession #: NP_004924.1
• NCBI GenBank Nucleotide #: NM_004933.2
• UniProt Accession #: P55291
• Molecular Weight: 88,916 Da
• NCBI Official Full Name: cadherin-15 preproprotein
• NCBI Official Synonym Full Names: cadherin 15, type 1, M-cadherin (myotubule)
• NCBI Official Symbol: CDH15
• NCBI Official Synonym Symbols: CDH3; CDHM; MCAD; MRD3; CDH14
• NCBI Protein Information: cadherin-15; cadherin-3; cadherin-14; muscle-cadherin
• UniProt Protein Name: Cadherin-15
• Protein Family: Cadherin
• UniProt Gene Name: CDH15
• UniProt Synonym Gene Names: CDH14; CDH3; M-cadherin
• UniProt Entry Name: CAD15_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH15: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24). Defects in CDH15 are the cause of mental retardation autosomal dominant type 3 (MRD3). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to membrane; plasma membrane; caveola; neuromuscular junction

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; muscle cell differentiation; positive regulation of muscle cell differentiation; homophilic cell adhesion; cell adhesion

Disease: Mental Retardation, Autosomal Dominant 3