Rabbit BLUF Polyclonal Antibody | anti-BLUF antibody

Rabbit anti-Escherichia coli (strain K12) BLUF Polyclonal Antibody

• Gene Names: bluF; ECK1150; JW1150; ycgF
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: BLUF; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) BLUF Polyclonal Antibody; BLUF Antibody; Blue light- and temperature-regulated antirepressor BluF; Blrp; bluF blrp ycgF b1163 JW1150; anti-BLUF antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-BLUF antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) bluF protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 16129126
• NCBI GeneID: 947592
• NCBI Accession #: NP_415681.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P75990
• Molecular Weight: 45,295 Da
• NCBI Official Full Name: anti-repressor for YcgE, blue light-responsive; FAD-binding; inactive c-di-GMP phosphodiesterase-like EAL domain protein
• NCBI Official Symbol: bluF
• NCBI Official Synonym Symbols: ECK1150; JW1150; ycgF
• NCBI Protein Information: anti-repressor for YcgE, blue light-responsive; FAD-binding; inactive c-di-GMP phosphodiesterase-like EAL domain protein
• UniProt Protein Name: Blue light- and temperature-regulated antirepressor BluF
• Protein Family: Blue light- and temperature-regulated antirepressor
• UniProt Gene Name: bluF
• UniProt Synonym Gene Names: Blrp

NCBI Description

YcgF has an N-terminal blue light-responsive FAD-binding BLUF domain. [More information is available at EcoGene: EG13887]. YcgF is a member of the family of blue-light sensing proteins that use FAD (BLUF). [More information is available at EcoCyc: G6603].

Uniprot Description

Binds to and releases the BluR repressor from its bound DNA target in a blue light-dependent (470 nm) fashion. A shift to low temperature also triggers a BluF-mediated relief of repression by BluR, suggesting BluF may serve as a thermometer. Blue light may act to increase the affinity of BluF for BluR, allowing it to be released from its operator. The protein has a reversible photocycle, and undergoes structural changes, probably in the EAL domain, in response to light.