HIS tag Peptide | HIS peptide
HIS tag (H20) Blocking Peptide
Synonyms
HIS tag; HIS tag (H20) Blocking Peptide; HIS peptide
Specificity
His (H20)is recommended for use by western blotting and immunoprecipitation.The suggested western blotting starting dilution is at 1:400.
Form/Format
Each vial contains 200 µg/ml of affinity purified rabbit IgG, His (H20) MBS443057, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin
Origin
His (H20) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide containing the 6x histidine tag.
Competitition studies
A blocking peptide is also available, MBS443057, for use in competition studies. Each vial contains 100 ug of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 ug BSA.
Preparation and Storage
Store this product at 4°C, do not freeze.
Related Product Information for HIS peptide
The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.
Product Categories/Family for HIS peptide
Similar Products
Product Notes
The HIS (Catalog #AAA443057) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HIS tag, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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