NEDD8 Activating Enzyme E1 Subunit 1 Recombinant Protein | NAE1 recombinant protein

Recombinant Human NEDD8 Activating Enzyme E1 Subunit 1

Cat. Num. AAA145030

• Gene Names: NAE1; HPP1; ula-1; APPBP1; A-116A10.1
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: NEDD8 Activating Enzyme E1 Subunit 1; Recombinant Human NEDD8 Activating Enzyme E1 Subunit 1; NAE1 Human; NEDD8 Activating Enzyme E1 Subunit 1 Human Recombinant; NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1; NAE1; APPBP1; HPP1; ula-1; A-116A10.1; NAE1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The NAE1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 200mM NaCl.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAQLGKL LKEQKYDRQL RLWGDHGQEA LESAHVCLIN ATATGTEILK NLVLPGIGSF TIIDGNQVSG EDAGNNFFLQ RSSIGKNRAE AAMEFLQELN SDVSGSFVEE SPENLLDNDP SFFCRFTVVV ATQLPESTSL RLADVLWNSQ IPLLICRTYG LVGYMRIIIK EHPVIESHPD NALEDLRLDK PFPELREHFQ SYDLDHMEKK DHSHTPWIVI IAKYLAQWYS ETNGRIPKTY KEKEDFRDLI RQGILKNENG APEDEENFEE AIKNVNTALN TTQIPSSIED IFNDDRCINI TKQTPSFWIL ARALKEFVAK EGQGNLPVRG TIPDMIADSG KYIKLQNVYR EKAKKDAAAV GNHVAKLLQS IGQAPESISE KELKLLCSNS AFLRVVRCRS LAEEYGLDTI NKDEIISSMD NPDNEIVLYL MLRAVDRFHK QQGRYPGVSN YQVEEDIGKL KSCLTGFLQE YGLSVMVKDD YVHEFCRYGA AEPHTIAAFL GGAAAQEVIK IITKQFVIFN NTYIYSGMSQ TSATFQL.
• Sequence Length: 528

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for NAE1 recombinant protein

Description: NAE1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 557 amino acids (1-534) and having a molecular mass of 62.7kDa.NAE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: NEDD8-activating enzyme E1 regulatory subunit (NAE1) is a member of the ubiquitin-activating E1 family. NAE1 binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is believed to have a role in the pathogenesis of Alzheimer's disease. NAE1 participates in a unique ubiquitinylation-related pathway involving the ubiquitin-like molecule NEDD8. Furthermore, the NAE1 protein is essential for cell cycle progression through the S/M checkpoint.

Product Categories/Family for NAE1 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 66363686
• NCBI GeneID: 8883
• NCBI Accession #: NP_001018169.1
• NCBI GenBank Nucleotide #: NM_001018159.1
• UniProt Accession #: Q13564; A6NCK0; A6NFN4; A8MU28; B2R700; B3KUP9
• Molecular Weight: 60,517 Da
• NCBI Official Full Name: NEDD8-activating enzyme E1 regulatory subunit isoform b
• NCBI Official Synonym Full Names: NEDD8 activating enzyme E1 subunit 1
• NCBI Official Symbol: NAE1
• NCBI Official Synonym Symbols: HPP1; ula-1; APPBP1; A-116A10.1
• NCBI Protein Information: NEDD8-activating enzyme E1 regulatory subunit; APP-BP1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; amyloid beta precursor protein-binding protein 1, 59kD; amyloid protein-binding protein 1; proto-oncogene protein 1; protooncogene protein 1
• UniProt Protein Name: NEDD8-activating enzyme E1 regulatory subunit
• Protein Family: NEDD8-activating enzyme E1 regulatory
• UniProt Gene Name: NAE1
• UniProt Synonym Gene Names: APPBP1; APP-BP1
• UniProt Entry Name: ULA1_HUMAN

NCBI Description

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; protein neddylation; neuron apoptosis; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction