Mouse Cadherin-2 (CDH2) ELISA Kit | CDH2 elisa kit

Mouse Cadherin-2 (CDH2) ELISA Kit

Cat. Num. AAA2800184

• Gene Names: Cdh2; CDHN; Ncad; N-CAD
• Reactivity: Mouse
• Synonyms: Cadherin-2 (CDH2); Mouse Cadherin-2 (CDH2) ELISA Kit; CD325; CDHN; CDw325; NCAD; N-cadherin 1; cadherin 2; N-cadherin (neuronal); type 1; calcium-dependent adhesion protein; neuronal; neural cadherin; CDH2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CDH2. No significant cross-reactivity or interference between Mouse CDH2 and analogues was observed.
• Sequence Length: 906

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CDH2 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate CDH2 in samples. An antibody specific for CDH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Cadherin 2, type 1, N-cadherin (neuronal), is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Reid and Hemperly (1990) obtained a full-length NCAD clone from a Kelly neuroblastoma library. The NCAD gene encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical.

NCBI and Uniprot Product Information

• NCBI GI #: 161760627
• NCBI GeneID: 12558
• NCBI Accession #: NP_031690.3
• NCBI GenBank Nucleotide #: NM_007664.5
• UniProt Accession #: P15116; Q64260; Q6GU11
• Molecular Weight: 99,796 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: Cdh2
• NCBI Official Synonym Symbols: CDHN; Ncad; N-CAD
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: Cdh2
• UniProt Synonym Gene Names: N-cadherin

NCBI Description

This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Mice lacking the encoded protein exhibit severe developmental defects resulting in embryonic death. [provided by RefSeq, Oct 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.