Rat anti-Mouse SPARC-like 1 Monoclonal Antibody | anti-SPARCL1 antibody
SPARC-like 1 (SPARCL1, Hevin, Mast9)
Gene Names
SPARCL1; SC1; PIG33
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography. Endotoxin: < 0.1EU/ug (LAL)
Synonyms
SPARC-like 1; Monoclonal Antibody; SPARC-like 1 (SPARCL1; Hevin; Mast9); Anti -SPARC-like 1 (SPARCL1; anti-SPARCL1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H30
Specificity
Recognizes mouse SPARC-like 1. Does not crossreact with recombinant mouse SPARC or recombinant human SPARC-like 1.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography. Endotoxin: < 0.1EU/ug (LAL)
Purified by Protein G affinity chromatography. Endotoxin: < 0.1EU/ug (LAL)
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-SPARCL1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1-2ug/ml. The dection limit for recombinant mouse SPARC-L1 is ~10ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse SPARC-L1 is ~10ng/well.
Dilution: Western Blot: 1-2ug/ml. The dection limit for recombinant mouse SPARC-L1 is ~10ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse SPARC-L1 is ~10ng/well.
Immunogen
Recombinant protein corresponding to aa17-650 of mouse SPARC-like 1, expressed in NSO cells (P70663).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SPARCL1 antibody
Mouse SPARC-like 1 (SPARC-L1), also known as SC1, Hevin, and MAST9, is a member of the SPARC family of extracellular glycoproteins. These proteins contain conserved structural domains including an N-terminal acidic region, followed by the follistatin-like domain, the Kazal-like domain, and the C-terminal Ca2+-binding E-F hand domain. The amino acid sequence of mouse mature SPARC-L1 is 89% and 67% identical to that of rat and human SPARC-L1, respectively.
Product Categories/Family for anti-SPARCL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,208 Da
NCBI Official Full Name
SPARC-like protein 1
NCBI Official Synonym Full Names
SPARC-like 1 (hevin)
NCBI Official Symbol
SPARCL1
NCBI Official Synonym Symbols
SC1; PIG33
NCBI Protein Information
SPARC-like protein 1; hevin; mast9; MAST 9; OTTHUMP00000161184; OTTHUMP00000219654; SPARC-like 1 (mast9, hevin); high endothelial venule protein; proliferation-inducing protein 33
UniProt Protein Name
SPARC-like protein 1
Protein Family
UniProt Gene Name
SPARCL1
UniProt Entry Name
SPRL1_HUMAN
Uniprot Description
SPARCL1: Belongs to the SPARC family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4q22.1
Cellular Component: proteinaceous extracellular matrix; extracellular space
Molecular Function: calcium ion binding
Biological Process: signal transduction
Research Articles on SPARCL1
Similar Products
Product Notes
The SPARCL1 sparcl1 (Catalog #AAA604094) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The SPARC-like 1 (SPARCL1, Hevin, Mast9) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPARC-like 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-2ug/ml. The dection limit for recombinant mouse SPARC-L1 is ~10ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse SPARC-L1 is ~10ng/well. Researchers should empirically determine the suitability of the SPARCL1 sparcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPARC-like 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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