Mouse Rad50 Monoclonal Antibody | anti-RAD50 antibody
Rad50 Monoclonal Antibody
This antibody recognizes human Rad50, a 153 kD protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex. Following DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
Positive control: Raji cells.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Uniprot Description
RAD50: a component of the Mre11-Rad50-NBS1 (MRN) complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Three alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold; EC 3.6.-.-; Hydrolase; DNA repair, damage
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; Mre11 complex; membrane; nuclear chromosome, telomeric region; pronucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding, bridging; protein binding; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; metal ion binding; 3'-5' exonuclease activity; ATP binding
Biological Process: positive regulation of kinase activity; telomere maintenance via telomerase; DNA repair; regulation of mitotic recombination; DNA catabolic process, endonucleolytic; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; DNA recombination; double-strand break repair; meiotic recombination; response to DNA damage stimulus; telomere maintenance
Disease: Nijmegen Breakage Syndrome-like Disorder
Research Articles on RAD50
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Product Notes
The RAD50 rad50 (Catalog #AAA190395) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rad50 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunoprecipitation. Immunoblotting, immunoprecipitation: use at 0.1-2 ug/ml. Positive control: Raji cells. Researchers should empirically determine the suitability of the RAD50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad50, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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