Mouse Heat Shock Protein 70, Heat Shock Cognate Protein 70 Monoclonal Antibody | anti-HSP70 antibody

Heat Shock Protein 70, Heat Shock Cognate Protein 70 (HSP70, HSC70)

Cat. Num. AAA633466

• Gene Names: HSP70; grp78
• Reactivity: Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat, Sheep, Xenopus, Yeast
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Heat Shock Protein 70; Heat Shock Cognate Protein 70; Monoclonal Antibody; Heat Shock Cognate Protein 70 (HSP70; HSC70); Anti -Heat Shock Protein 70; anti-HSP70 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat, Sheep, Xenopus, Yeast
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7L12
• Specificity: Recognizes human HSP70/HSC70. Detects 72 and 73kD proteins corresponding to the molecular mass of inducible HSP70 and HSC70 on SDS PAGE immunoblots. Species Crossreactivity: Mouse, rat, sheep, dog, beluga, bovine, fish (carp, rainbow trout, chinook/chum salmon) guinea pig, scallop pig, hamster, rabbit, chicken, Xenopus, Drosophila, and yeast.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-HSP70 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml was sufficient for detection of hsp70/Hsc70 in 20ug of HeLa lysate.
• Immunogen: Chicken Hsp70/Hsp90 complex
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HSP70 antibody

Hsp70 genes encode abundant heat-inducible 70-kD hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kD which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Product Categories/Family for anti-HSP70 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 112984012
• NCBI GeneID: 692373
• NCBI Accession #: NP_001036837.1
• NCBI GenBank Nucleotide #: NM_001043372.1
• NCBI Official Full Name: heat shock 70 kD protein cognate
• NCBI Official Symbol: HSP70
• NCBI Official Synonym Symbols: grp78
• NCBI Protein Information: heat shock 70 kD protein cognate; heat shock protein 70; heat shock protein 70-3; glucose-regulated protein 78
• Protein Family: Heat shock 70 kDa protein