Rat anti-Human Estrogen Receptor-alpha Monoclonal Antibody | anti-ER-alpha antibody

Anti-Human Estrogen Receptor-alpha Antibody

Cat. Num. AAA419342

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: Estrogen Receptor-alpha; Monoclonal Antibody; Anti-Human Estrogen Receptor-alpha Antibody; anti-ER-alpha antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 444

• Applicable Applications for anti-ER-alpha antibody: Western Blot (WB), ELISA (EIA)
• Immunogen: Purified estrogen receptor from MCF-7 cells
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Related Product Information for anti-ER-alpha antibody

The human estrogen receptor alpha protein is 66 kDa, though multiple isoforms ranging from 36-66 kDa have been described. Estrogen receptor is an important regulator of growth and differentiation in the mammary gland.

NCBI and Uniprot Product Information

• NCBI GI #: 5821726
• NCBI GeneID: 2099
• Molecular Weight: 35,547 Da
• NCBI Official Full Name: estrogen receptor alpha, partial
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.