APRIL active protein

Human APRIL

Cat. Num. AAA691562

• Gene Names: TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
• Reactivity: Human
• Purity: >95% by SDS-PAGE and HPLC analysis
• Synonyms: APRIL; Human APRIL; Recombinant Human April; APRIL active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: >95% by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQA QGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF R CIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPH GTF LGFVKL
• Sequence Length: 222

• Endotoxin Level: < 0.1 ng per ug of April
• Biological Activity: Tested by its ability to induce IgM secretion by CpG/CD40L treated human naive B cells, at a concentration of 500ng/ml.

Related Product Information for APRIL active protein

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.

Product Categories/Family for APRIL active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 310750387
• NCBI GeneID: 8741
• NCBI Accession #: NP_001185552.1
• NCBI GenBank Nucleotide #: NM_001198623.1
• UniProt Accession #: O75888; Q541E1; Q5U0G8; Q96HV6; Q9P1M8; Q9P1M9; A8MYD5; B4DVT2
• Molecular Weight: 16.3 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13 isoform zeta
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13
• NCBI Official Symbol: TNFSF13
• NCBI Official Synonym Symbols: APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13
• UniProt Gene Name: TNFSF13
• UniProt Synonym Gene Names: APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1
• UniProt Entry Name: TNF13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

Function: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Ref.12

Subunit structure: Homotrimer

Potential.

Subcellular location: Secreted Ref.13.

Tissue specificity: Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.

Induction: Down-regulated by phorbol myristate acetate/ionomycin treatment.

Post-translational modification: The precursor is cleaved by furin. Ref.13

Sequence similarities: Belongs to the tumor necrosis factor family.