Ansamitocin p-3, Inhibitor

Ansamitocin p-3

Cat. Num. AAA5756791

• Purity: 98.00%
• Synonyms: Ansamitocin p-3; Antibiotic C 15003P3; NSC292222; Maytansinol isobutyrate; inhibitor

• Purity/Purification: 98.00%
• Form/Format: Solid. Powder

• Solubility: DMSO: 100mg/ml (157.45mM); Ethanol: 55mg/ml (86.6mM); (<1mg/ml refers to the product slightly soluble or insoluble)
• Formula: C32H43ClN2O9
• CAS Number: 66584-72-3
• Target & IC50: Others
• Cell Research: Cell lines: A31 and KB. Concentrations: Dissolved in DMSO, final concentrations ~10uM. Incubation Time: ~24 hours. Method: After synchronized, cells are exposed to various Ansamitocin p-3 concentrations for ~24 hours. Cells are labeled with [3H]thymidine (5Ci/mM, 1 uCi/mL) in 1mL medium. After pulse-labeling at 37 degree C for 1 hour, the cells on coverslips are fixed with methanol:acetic acid (3:1). The acidsoluble fraction is washed out from the cells, and a liquid scintillation counter is used to determine the radio activity of each coverslip.
• Kinase Assay: Polymerization inhibition assay: After the addition of 1M Tris buffer (pH 8.4, for blank) or 100uL of various concentrations of Ansamitocin p-3 solution (GTP minus MES buffer), to 400uL of bovine tubulin solution (1mg/mL in cold MES buffer), maintained at 0 degree C for 10 to 15 minutes, the mixture is warmed in a water bath at 37 degree C for 30 to 60 minutes. Tubulin polymerization is followed by an increase in turbidity of the mixture during warming. The turbidity measurement is performed at 460nM with a Hitachi type 101 spectrophotometer.
• Preparation and Storage: Store at -20 degree C for 3 years powder; -80 degree C for 2 years in solvent

Related Product Information for Ansamitocin p-3, inhibitor

Ansamitocin p-3 (Maytansinol isobutyrate, NSC292222) is a potent tubulin polymerization inhibitor with IC50 of 3.4uM.

References

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Jubina B Venghateri, et al. PLoS One. 2013 Oct 4;8(10):e75182.