Rat DNA-binding protein inhibitor ID-2 ELISA Kit | ID2 elisa kit

Rat DNA-binding protein inhibitor ID-2 (ID2) ELISA Kit

Cat. Num. AAA285809

• Gene Names: Id2; Ac2-300
• Reactivity: Rat
• Synonyms: DNA-binding protein inhibitor ID-2; Rat DNA-binding protein inhibitor ID-2 (ID2) ELISA Kit; GIG8; ID2A; ID2H; MGC26389; bHLHb26; DNA-binding protein inhibitor ID2|OTTHUMP00000140258|OTTHUMP00000200297|cell growth-inhibiting gene 8|helix-loop-helix protein ID2|inhibitor of DNA binding 2|inh; ID2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ID2. No significant cross-reactivity or interference between Rat ID2 and analogues was observed.
• Sequence Length: 134

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ID2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ID2 in samples. An antibody specific for ID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6981072
• NCBI GeneID: 25587
• NCBI Accession #: NP_037192.1
• NCBI GenBank Nucleotide #: NM_013060.3
• UniProt Accession #: P41137
• Molecular Weight: 14,989 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-2
• NCBI Official Synonym Full Names: inhibitor of DNA binding 2
• NCBI Official Symbol: Id2
• NCBI Official Synonym Symbols: Ac2-300
• NCBI Protein Information: DNA-binding protein inhibitor ID-2
• UniProt Protein Name: DNA-binding protein inhibitor ID-2
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: Id2
• UniProt Synonym Gene Names: Id-2

NCBI Description

a helix-loop-helix protein that lacks a DNA binding domain; involved in transcription regulation [RGD, Feb 2006]

Uniprot Description

ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: chromatin; cytoplasm; cytosol; nucleus; protein complex

Molecular Function: ion channel binding; protein binding; protein dimerization activity; protein heterodimerization activity

Biological Process: adult locomotory behavior; bundle of His development; cell development; cell maturation; cell morphogenesis involved in neuron differentiation; cellular response to lithium ion; cellular senescence; circadian regulation of gene expression; circadian rhythm; embryonic digestive tract morphogenesis; entrainment of circadian clock; entrainment of circadian clock by photoperiod; enucleate erythrocyte differentiation; heart development; leukocyte differentiation; locomotor rhythm; mammary gland epithelial cell proliferation; membranous septum morphogenesis; metanephros development; natural killer cell differentiation; negative regulation of B cell differentiation; negative regulation of DNA binding; negative regulation of gene expression; negative regulation of neural precursor cell proliferation; negative regulation of neuron differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of osteoblast differentiation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron differentiation; neuron fate commitment; olfactory bulb development; oligodendrocyte development; Peyer's patch development; positive regulation of astrocyte differentiation; positive regulation of blood pressure; positive regulation of cell cycle; positive regulation of cell cycle arrest; positive regulation of cell proliferation; positive regulation of erythrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of gene expression; positive regulation of macrophage differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-templated; regulation of circadian rhythm; regulation of G1/S transition of mitotic cell cycle; regulation of lipid metabolic process; thigmotaxis; transcription, DNA-dependent