Factor XIa Protein
Factor XIa, Human
Purity
Affinity PurifiedFactor XIa is prepared by activating purified factor XI with factor XIIa. Following activation, the mixture is passed over a column of immobilized anti-factor XII to remove the factor XIIa, and further purified on heparin-Sepharose. P
Synonyms
Factor XIa; Human; Factor XIa protein
Host
Human plasma
Purity/Purification
Affinity Purified
Factor XIa is prepared by activating purified factor XI with factor XIIa. Following activation, the mixture is passed over a column of immobilized anti-factor XII to remove the factor XIIa, and further purified on heparin-Sepharose. P
Factor XIa is prepared by activating purified factor XI with factor XIIa. Following activation, the mixture is passed over a column of immobilized anti-factor XII to remove the factor XIIa, and further purified on heparin-Sepharose. P
Form/Format
The factor XIa is supplied in 50% glycerol/H2O.
Extinction Coefficient
E1%1cm, 280nm=13.4 (Inferred from the zymogen, factor XI) (2)
Mode of Action
A serine protease that converts factor IX to factor IXa
Structure
Two apparently identical heavy chains (Mr~50,000) and two apparently identical light chains (Mr~30,000) held together by disulfide bonds. Each light chain contains a catalytic domain (1,3).
Percent Carbohydrate
5% (1)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Factor XIa protein
Proteolytic Activation of Factor XI: Although factor XI can bind independently to negatively charged surfaces, proteolysis by factor XIIa can only occur in the presence of surface-bound high molecular weight kininogen (HMWKa). The bound HMWKa is susceptible to proteolysis by the newly generated factor XIa which results in a loss of its biological activity (HMWKi). Factor XIa catalyzes the proteolytic conversion of factor IX to factor IXa either in solution phase or when bound to a negatively charged surface.
Factor XIa, like its precursor factor XI, remains in complex with HMWK (4). In complex form, factor XIa/HMWK is capable of activating factor XII to factor XIIa and prekallikrein to kallikrein. Additionally, in the complexed form, factor XIa is less affected by protease inhibitors (5). The major plasma inhibitor of factor XIa is a1-antitrypsin and then to a much lesser extent, antithrombin-III (5,6). While in the complexed form, factor XIa may catalyze the proteolysis of HMWK, yielding a biologically inactive form of HMWK (HMWKi), thus allowing dissociation of the Factor XIa/HMWKi complex. Either free factor XIa or the factor XIa/HMWK complex catalyzes the proteolytic conversion of factor IX to factor IXa.
Factor XIa, like its precursor factor XI, remains in complex with HMWK (4). In complex form, factor XIa/HMWK is capable of activating factor XII to factor XIIa and prekallikrein to kallikrein. Additionally, in the complexed form, factor XIa is less affected by protease inhibitors (5). The major plasma inhibitor of factor XIa is a1-antitrypsin and then to a much lesser extent, antithrombin-III (5,6). While in the complexed form, factor XIa may catalyze the proteolysis of HMWK, yielding a biologically inactive form of HMWK (HMWKi), thus allowing dissociation of the Factor XIa/HMWKi complex. Either free factor XIa or the factor XIa/HMWK complex catalyzes the proteolytic conversion of factor IX to factor IXa.
Product Categories/Family for Factor XIa protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
160,000
NCBI Official Full Name
follipsin, serine proteinase, FOL32K=plasma kallikrein and factor XIa light chain homolog {N-terminal}
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Product Notes
The Factor XIa (Catalog #AAA634636) is a Protein produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Factor XIa, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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