Troponin I-Cardiac >95% Pure Native Protein | TNNI3 native protein

Troponin I-Cardiac >95% Pure

Cat. Num. AAA318328

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• Applications: ELISA, Western Blot
• Purity: >98% pure (SDS-PAGE); Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.
• Synonyms: Troponin I-Cardiac >95% Pure; Cardiac Troponin I (cTnI); >95% Pure; TNNI3 native protein

• Host: Human heart tissue
• Specificity: Troponin I-Cardiac >95% Pure
• Purity/Purification: >98% pure (SDS-PAGE); Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.
• Form/Format: Purified, Lyophilized; Reconstitute in urea/Tris buffer, pH 7.5 (7M urea, 5mM EDTA, 15mM mercaptoethanol, 20mM Tris). Solubility is limited at neutral pH and physiological salt concentration.
• Concentration: Determined prior to lyophilization (OD280nm, E^0.1% = 0.42); This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).; It is recommended that the product should be reconstituted in urea/Tris buffer, pH 7.5 (7M (varies by lot)

• Applicable Applications for TNNI3 native protein: EIA/ELISA, Western Blot
• Inactivation: Not applicable
• Buffer: Lyophilized from 0.01M HCl
• Preservative: No
• Lyophilized: Yes
• Warnings: A blood sample form the tissue donor tested negative for HIV 1, HIV 2, HCV antibodies and HBsAg and Syphilis. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as
• Preparation and Storage: Store lyophilized product at -20 degree C. After reconstitution, store at -20 degree C. Avoid multiple freeze/thaw cycles.

Product Categories/Family for TNNI3 native protein

Purified Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429; Q6FGU5; Q6FGX2
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I type 3 (cardiac)
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; MGC116817
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Uniprot Description

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Subunit structure: Binds to actin and tropomyosin. Interacts with TRIM63. Ref.7

Involvement in disease: Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [

MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Ref.10 Ref.11 Ref.13 Ref.15 Ref.17Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [

MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [

MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Ref.16Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [

MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Sequence similarities: Belongs to the troponin I family.