Human PSA ELISA Kit
Free PSA ELISA, 96 well
Reactivity
Human
Synonyms
PSA; Free PSA ELISA; 96 well; PSA elisa kit
Reactivity
Human
Specificity
The antibodies in the MyBioSource Estrone-3-Sulfate equine ELISA procedure are highly specific for estrone-3-sulfate, with low cross-reactivities to other steroids as listed below
Samples
Mare Serum
Assay Type
Competitive
Sensitivity
0.14 ng/ml.
Preparation and Storage
2 - 8 degree C
Related Product Information for PSA elisa kit
Intended Uses: The MyBioSource Estrone-3-Sulfate equine is a competitive enzyme immunoassay for the measurement of estrone-3-sulfate in mare serum. For Research Use Only,.
Principle of the Assay: The MyBioSource Estrone-3-Sulfate equine ELISA test kit is a solid phase enzyme immunoassay (ELISA) in the microplate format, designed for the measurement of estrone-3-sulfate. The microplate is coated with a polyclonal antibody specific for estrone-3-sulfate. Calibrators and samples as well as a biotin-labeled estrone-3-sulfate are pipetted into the antibody coated microplate. During one hour incubation the estrone-3-sulfate of the calibrator/sample competes with the biotin-labeled estrone-sulfate for the binding sites of the antibody fixed on the inner surface of the wells. Afterwards, horseradish peroxidase-labeled streptavidine is added. During an 30 minutes incubation, this enzyme-labeled streptavidine binds to the biotinylated estrone-3-sulfate. All wells are washed to remove excess of non-bound enzyme-labeled streptavidine. A chromogenic substrate, TMB (3,3',5,5'-Tetra-Methyl-Benzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensity is inversely related to the concentration of estrone-3-sulfate present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600-690 nm reference filter is recommended.
Principle of the Assay: The MyBioSource Estrone-3-Sulfate equine ELISA test kit is a solid phase enzyme immunoassay (ELISA) in the microplate format, designed for the measurement of estrone-3-sulfate. The microplate is coated with a polyclonal antibody specific for estrone-3-sulfate. Calibrators and samples as well as a biotin-labeled estrone-3-sulfate are pipetted into the antibody coated microplate. During one hour incubation the estrone-3-sulfate of the calibrator/sample competes with the biotin-labeled estrone-sulfate for the binding sites of the antibody fixed on the inner surface of the wells. Afterwards, horseradish peroxidase-labeled streptavidine is added. During an 30 minutes incubation, this enzyme-labeled streptavidine binds to the biotinylated estrone-3-sulfate. All wells are washed to remove excess of non-bound enzyme-labeled streptavidine. A chromogenic substrate, TMB (3,3',5,5'-Tetra-Methyl-Benzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensity is inversely related to the concentration of estrone-3-sulfate present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600-690 nm reference filter is recommended.
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Product Notes
The Human PSA (Catalog #AAA494876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494876 ELISA Kit recognizes Human PSA. It is sometimes possible for the material contained within the vial of "PSA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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