Human KIM-1 ELISA Kit
Human KIM-1 ELISA Kit
Reactivity
Human
Synonyms
KIM-1; Human KIM-1 ELISA Kit; Human KIM-1; KIM-1 elisa kit
Reactivity
Human
Samples
Immunoperoxidase Assay for Determination of Kim-1 in Human Samples
Assay Type
Sandwich
Intended Uses
The Kidney Injury Molecule (KIM-1) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring KIM-1 in biological samples of Humans.
Preparation and Storage
Store at 4 degree C.
Related Product Information for KIM-1 elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti- KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.
Background: KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.
Product Categories/Family for KIM-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
kidney injury molecule-1
Uniprot Description
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair ().
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Product Notes
The Human KIM-1 (Catalog #AAA564161) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564161 ELISA Kit recognizes Human KIM-1. It is sometimes possible for the material contained within the vial of "KIM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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