Human Glucocorticoid (GC) ELISA Kit | GC elisa kit

Human Glucocorticoid (GC) ELISA Kit

Cat. Num. AAA287644

• Reactivity: Human
• Synonyms: Glucocorticoid (GC); Human Glucocorticoid (GC) ELISA Kit; DBP; DBP/GC; GRD3; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein; GC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GC. No significant cross-reactivity or interference between Human GC and analogues was observed.

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Range: 18.52-1500 pg/mL
• Sensitivity: 7.54 pg/mL
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GC elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate GC in samples. An antibody specific for GC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Glucagon has a major role in maintaining normal concentrations of glucose in blood, and is often described as having the opposite effect of insulin. That is, glucagon has the effect of increasing blood glucose levels. Glucagon is a linear peptide of 29 amino acids. Its primary sequence is almost perfectly conserved among vertebrates, and it is structurally related to the secretin family of peptide hormones.
Glucagon is synthesized as proglucagon and proteolytically processed to yield glucagon within alpha cells of the pancreatic islets. Proglucagon is also expressed within the intestinal tract, where it is processed not into glucagon, but to a family of glucagon-like peptides (enteroglucagon).