Human Elongation factor 1-alpha 2 (EEF1A2) ELISA Kit | EEF1A2 elisa kit
Human Elongation factor 1-alpha 2 (EEF1A2) ELISA Kit
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
Reactivity
Human
Synonyms
Elongation factor 1-alpha 2 (EEF1A2); Human Elongation factor 1-alpha 2 (EEF1A2) ELISA Kit; EEF1AL; EF-1-alpha-2; EF1A; FLJ41696; HS1; STN; STNL; OTTHUMP00000031776|elongation factor 1-alpha 2|elongation factor-1 alpha|statin S1|statin-like; EEF1A2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human EEF1A2. No significant cross-reactivity or interference between Human EEF1A2 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate EEF1A2 in samples. An antibody specific for EEF1A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEEF1A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EEF1A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EEF1A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
Related Product Information for EEF1A2 elisa kit
EEF1a2is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.EF1-alpha is distributed widely, although expression is very low or undetectable in skeletal and cardiac muscle postnatally, and expression in the brain declines between embryonic life and adulthood in rats. EF1-alpha-2 is found exclusively in terminally differentiated cells of skeletal muscle, heart, and certain areas of the brain. In those tissues where both genes are expressed, Eef1a2 is consistently more highly expressed than Eef1a.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50kDa
NCBI Official Full Name
elongation factor 1-alpha 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 2
NCBI Official Symbol
EEF1A2
NCBI Official Synonym Symbols
HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
NCBI Protein Information
elongation factor 1-alpha 2; eEF1A-2; statin S1; statin-S1; elongation factor-1 alpha; eukaryotic elongation factor 1 A-2
UniProt Protein Name
Elongation factor 1-alpha 2
UniProt Gene Name
EEF1A2
UniProt Synonym Gene Names
EEF1AL; STN; EF-1-alpha-2; eEF1A-2
UniProt Entry Name
EF1A2_HUMAN
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Product Notes
The Human EEF1A2 eef1a2 (Catalog #AAA2802227) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2802227 ELISA Kit recognizes Human EEF1A2. It is sometimes possible for the material contained within the vial of "Elongation factor 1-alpha 2 (EEF1A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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