MFI2 Cell Lysate | MELTF cell lysate
Mouse MFI2 HEK293 Overexpression Lysate
Gene Names
Mfi2; MTf; CD228
Applications
Western Blot
Synonyms
MFI2; Mouse MFI2 HEK293 Overexpression Lysate; Mouse MFI2/CD228/melanotransferrin HEK293 Cell Lysate (WB positive control); melanotransferrin; Mouse CD228 Overexpression Lysate; Mouse MTf Overexpression Lysate; MELTF cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
700aa
Sequence
Met1-Gln708
Applicable Applications for MELTF cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse MFI2 (Q9R0R1?) (Met 1-Gln 708), without the propeptide, was fused with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MELTF cell lysate
MELTF (Melanotransferrin, also known as MFI2) is a Protein Coding gene. The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. MELTF is broadly expressed in the salivary gland, kidney, and other tissues. The long non-coding RNA MFI2 antisense RNA is overexpressed in human cancer tissues and its increased expression is associated with the occurrence and metastasis of cancer. lncRNA MFI2 could promote proliferation and migration of osteosarcoma cells by regulating FOXP4 expression, which suggested critical roles of lncRNA MFI2 and FOXP4 in the occurrence and development of human osteosarcoma. Diseases associated with MELTF include Osteogenic Sarcoma and Melanotic Neuroectodermal Tumor.
This Mouse MFI2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MFI2 protein from the overexpression lysate was verified.
This Mouse MFI2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MFI2 protein from the overexpression lysate was verified.
References
Richardson DR. (2000) The role of the membrane-bound tumour antigen, melanotransferrin (p97), in iron uptake by the human malignant melanoma cell. Eur J Biochem. 267(5): 1290-8.Kim DK, et al. (2001) Serum melanotransferrin, p97 as a biochemical marker of Alzheimer's disease. Neuropsychopharmacology. 25(1): 84-90.Rothenberger S, et al. (1996) Coincident expression and distribution of melanotransferrin and transferrin receptor in human brain capillary endothelium. Brain Res. 712(1): 117-21.Baker EN, et al. (1992) Human melanotransferrin (p97) has only one functional iron-binding site. FEBS Lett. 298(2-3): 215-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,293 Da
NCBI Official Full Name
melanotransferrin
NCBI Official Synonym Full Names
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
NCBI Official Symbol
Mfi2
NCBI Official Synonym Symbols
MTf; CD228
NCBI Protein Information
melanotransferrin; membrane-bound transferrin-like protein p97
UniProt Protein Name
Melanotransferrin
Protein Family
UniProt Gene Name
Mfi2
UniProt Synonym Gene Names
Mtf; MTf
UniProt Entry Name
TRFM_MOUSE
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Product Notes
The MELTF mfi2 (Catalog #AAA8116979) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 700aa. AAA Biotech's MFI2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MELTF mfi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gln70 8. It is sometimes possible for the material contained within the vial of "MFI2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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