TCCR Cell Lysate | IL27Ra cell lysate
Human TCCR HEK293 Overexpression Lysate
Gene Names
IL27RA; CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
Applications
Western Blot
Synonyms
TCCR; Human TCCR HEK293 Overexpression Lysate; Human IL27Ra/TCCR/WSX1 HEK293 Cell Lysate (WB positive control); interleukin 27 receptor; alpha; Human CRL1 Overexpression Lysate; Human IL-27RA Overexpression Lysate; Human IL27R Overexpression Lysate; Human TCCR Overexpression Lysate; Human WSX1 Overexpression Lysate; Human zcytor1 Overexpression Lysate; IL27Ra cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
722aa
Sequence
Met1-Arg514
Applicable Applications for IL27Ra cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain (Met 1-Arg 514) of human IL27Ralpha (NP_004834.1) was fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL27Ra cell lysate
The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit, and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the beta2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4(+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.
This Human TCCR overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TCCR protein from the overexpression lysate was verified.
This Human TCCR overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TCCR protein from the overexpression lysate was verified.
Product Categories/Family for IL27Ra cell lysate
References
Pflanz S, et al.. (2004) WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27. J Immunol. 172(4): 2225-31.Yoshida H, et al.. (2001) WSX-1 is required for the initiation of Th1 responses and resistance to L. major infection. Immunity. 215(4): 569-78. Villarino A, et al.. (2003) The IL-27R (WSX-1) is required to suppress T cell hyperactivity during infection. Immunity. 19(5): 645-55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,474 Da
NCBI Official Full Name
interleukin-27 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 27 receptor, alpha
NCBI Official Symbol
IL27RA
NCBI Official Synonym Symbols
CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
NCBI Protein Information
interleukin-27 receptor subunit alpha; IL-27R-alpha; IL-27R subunit alpha; cytokine receptor WSX-1; cytokine receptor-like 1; class I cytokine receptor; IL-27 receptor subunit alpha; T-cell cytokine receptor type 1; type I T-cell cytokine receptor
UniProt Protein Name
Interleukin-27 receptor subunit alpha
Protein Family
UniProt Gene Name
IL27RA
UniProt Synonym Gene Names
CRL1; TCCR; WSX1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
UniProt Entry Name
I27RA_HUMAN
Uniprot Description
IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: integral to plasma membrane
Molecular Function: interleukin-27 receptor activity; transmembrane receptor activity
Biological Process: defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; positive regulation of T-helper 1 type immune response; negative regulation of T-helper 2 type immune response; immune response; regulation of isotype switching to IgG isotypes
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Product Notes
The IL27Ra il27ra (Catalog #AAA8113900) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 722aa. AAA Biotech's TCCR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL27Ra il27ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Arg51 4. It is sometimes possible for the material contained within the vial of "TCCR, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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