TREML2 cell lysate
Human TREML2 HEK293 Overexpression Lysate
Gene Names
TREML2; TLT2; TLT-2; C6orf76; FLJ13693; MGC149715; MGC149716; dJ238O23.1
Applications
Western Blot
Synonyms
TREML2; Human TREML2 HEK293 Overexpression Lysate; Human TREML2/TLT2 HEK293 Cell Lysate (WB positive control); triggering receptor expressed on myeloid cells like 2; Human C6orf76 Overexpression Lysate; Human dJ238O23.1 Overexpression Lysate; Human FLJ13693 Overexpression Lysate; Human MGC149715 Overexpression Lysate; Human MGC149716 Overexpression Lysate; Human TLT-2 Overexpression Lysate; Human TLT2 Overexpression Lysate; Human UNQ6268/PRO20473 Overexpression Lysate; TREML2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
261aa
Sequence
Met1-Ser268
Applicable Applications for TREML2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TREML2 (NP_079083.2) extracellular domain (Met 1-Ser 268) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TREML2 cell lysate
Trem-like transcript 2 protein, also known as Triggering receptor expressed on myeloid cells-like protein 2, TREML2 and TLT2, is a single-pass type I membrane protein that contains one Ig-like V-type (immunoglobulin-like) domain. TREML2 is detected in cultured B cells, T cell leukemia and monocyte leukemia. TREML2 is expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation. TREML2 is a cell surface receptor that may play a role in the innate and adaptive immune response. TREML2 acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation. Murine B7-H3 is specifically bound to Triggering receptor expressed on myeloid cells (TREM)-like transcript 2 (TLT-2, TREML2). TREML2 was expressed on CD8(+) T cells constitutively and on activated CD4(+) T cells. Stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production of CD8(+) T cells. Transduction of TREML2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3. There may be a direct interaction between B7-H3 and TREML2 that preferentially enhances CD8(+) T cell activation.
This Human TREML2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TREML2 protein from the overexpression lysate was verified.
This Human TREML2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TREML2 protein from the overexpression lysate was verified.
Product Categories/Family for TREML2 cell lysate
References
Mungall A.J. et al., 2003, Nature. 425: 805-11. Clark HF. et al., 2003, Genome Res. 13: 2265-70. The MGC Project Team. et al., 2004, Genome Res. 14:2121-7. Hashiguchi M., et al., 2008, Proc. Natl.Acad. Sci. USA.105:10495-500. Leitner, J. et al., 2009, Eur J Immunol. 39 (7):1754-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,127 Da
NCBI Official Full Name
trem-like transcript 2 protein
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells-like 2
NCBI Official Symbol
TREML2
NCBI Official Synonym Symbols
TLT2; TLT-2; C6orf76; FLJ13693; MGC149715; MGC149716; dJ238O23.1
NCBI Protein Information
trem-like transcript 2 protein; OTTHUMP00000017974; triggering receptor expressed on myeloid cells-like protein 2
UniProt Protein Name
Trem-like transcript 2 protein
Protein Family
UniProt Gene Name
TREML2
UniProt Synonym Gene Names
C6orf76; TLT2
UniProt Entry Name
TRML2_HUMAN
NCBI Description
TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties (Allcock et al., 2003 [PubMed 12645956]).[supplied by OMIM]
Uniprot Description
TREML2: Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cell surface; integral to membrane; plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: T cell activation
Research Articles on TREML2
Similar Products
Product Notes
The TREML2 treml2 (Catalog #AAA8114631) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 261aa. AAA Biotech's TREML2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TREML2 treml2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser26 8. It is sometimes possible for the material contained within the vial of "TREML2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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