TLT-1/TREML1 Cell Lysate | TREML1 cell lysate
Human TLT-1/TREML1 HEK293 Overexpression Lysate
Gene Names
TREML1; TLT1; TLT-1; PRO3438; GLTL1825; dJ238O23.3
Applications
Western Blot
Synonyms
TLT-1/TREML1; Human TLT-1/TREML1 HEK293 Overexpression Lysate; Human TREML1/TLT-1 HEK293 Cell Lysate (WB positive control); triggering receptor expressed on myeloid cells like 1; Human dJ238O23.3 Overexpression Lysate; Human GLTL1825 Overexpression Lysate; Human MGC119173 Overexpression Lysate; Human PRO3438 Overexpression Lysate; Human TLT-1 Overexpression Lysate; Human TLT1 Overexpression Lysate; Human UNQ1825/PRO3438 Overexpression Lysate; TREML1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
158aa
Sequence
Met1-Pro162
Applicable Applications for TREML1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human TREML1 isoform 1 (Q86YW5-1) extracellular domain (Met 1-Pro 162) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TREML1 cell lysate
Trem-like transcript 1 protein, also known as Triggering receptor expressed on myeloid cells-like protein 1, TREML1 and TLT-1, is a cytoplasm and single-pass type I membrane protein. TREML1/TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1/TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain, its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte/macrophages, and dendritic cells, via their association with DAP12. TREML1/TLT-1 is prepackaged, along with CD62P, into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1/TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury.
This Human TLT-1/TREML1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TLT-1/TREML1 protein from the overexpression lysate was verified.
This Human TLT-1/TREML1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TLT-1/TREML1 protein from the overexpression lysate was verified.
Product Categories/Family for TREML1 cell lysate
References
Washington A.V., et al., 2002, Blood 100:3822-4. Allcock R.J.N., et al., 2003, Eur. J. Immunol. 33:567-77. Washington A.V., et al., 2004, Blood 104:1042-7. Barrow A.D., et al., 2004, J. Immunol. 172:5838-42. Gattis J.L., et al., 2006, J. Biol. Chem. 281:13396-403.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,731 Da
NCBI Official Full Name
trem-like transcript 1 protein isoform b
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells like 1
NCBI Official Symbol
TREML1
NCBI Official Synonym Symbols
TLT1; TLT-1; PRO3438; GLTL1825; dJ238O23.3
NCBI Protein Information
trem-like transcript 1 protein
UniProt Protein Name
Trem-like transcript 1 protein
Protein Family
UniProt Gene Name
TREML1
UniProt Synonym Gene Names
TLT1; TLT-1
UniProt Entry Name
TRML1_HUMAN
NCBI Description
This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. The encoded protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012]
Uniprot Description
TREML1: Cell surface receptor that may play a role in the innate and adaptive immune response. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: plasma membrane
Biological Process: regulation of immune response
Research Articles on TREML1
Similar Products
Product Notes
The TREML1 treml1 (Catalog #AAA8114829) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 158aa. AAA Biotech's TLT-1/TREML1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TREML1 treml1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro16 2. It is sometimes possible for the material contained within the vial of "TLT-1/TREML1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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