GPX7 cell lysate
Human GPX7 HEK293 Overexpression Lysate
Gene Names
GPX7; GPX6; CL683; GPx-7; NPGPx; GSHPx-7
Applications
Western Blot
Synonyms
GPX7; Human GPX7 HEK293 Overexpression Lysate; Human GPX7/Glutathione Peroxidase 7 HEK293 Cell Lysate (WB positive control); glutathione peroxidase 7; Human CL683 Overexpression Lysate; Human GPx-7 Overexpression Lysate; Human GPX6 Overexpression Lysate; Human GSHPx-7 Overexpression Lysate; Human NPGPx Overexpression Lysate; Human UNQ469/PRO828 Overexpression Lysate; GPX7 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
408aa
Sequence
Met1-Leu187
Applicable Applications for GPX7 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human GPX7 (NP_056511.2) (Met1-Leu187) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GPX7 cell lysate
GPX7 gene contains 3 distinct gt-ag introns. Transcription produces 4 different mRNAs, 3 alternatively spliced variants, and 1 unspliced form. There are 5 validated alternative polyadenylation sites. The mRNAs appear to differ by overlapping exons with different boundaries. GPX7 is an enzyme. It has molecular functions (glutathione peroxidase activity, oxidoreductase activity) and to localize in various compartments (extracellular space, extracellular region). GPX7 gene has been proposed to participate in pathways (Arachidonic acid metabolism, Glutathione metabolism), and processes (oxidation-reduction, response to oxidative stress). GPX7 modulates the bone turnover after ovariectomy in rats, it does not compensate for the action of estrogen after ovariectomy in rats. It has been shown that three mAbs (GPX7, GPX22, and GPZ35) inhibit IL-6-mediated biological responses such as Ig production in a human B cell line and proliferative responses of a human Lennert's lymphoma-derived T cell line, a human myeloma cell line, and a mouse pro-B cell line-derived transfectant expressing human gp130.
This Human GPX7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPX7 protein from the overexpression lysate was verified.
This Human GPX7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GPX7 protein from the overexpression lysate was verified.
References
Rual JF, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8.Gregory SG, et al. (2006) The DNA sequence and biological annotation of human chromosome 1. Nature. 441(7091):315-21.Lim J, et al. (2006) A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell. 125(4):801-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,996 Da
NCBI Official Full Name
glutathione peroxidase 7
NCBI Official Synonym Full Names
glutathione peroxidase 7
NCBI Official Symbol
GPX7
NCBI Official Synonym Symbols
GPX6; CL683; GPx-7; NPGPx; GSHPx-7
NCBI Protein Information
glutathione peroxidase 7; glutathione peroxidase 6; non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase
UniProt Protein Name
Glutathione peroxidase 7
Protein Family
UniProt Gene Name
GPX7
UniProt Synonym Gene Names
GPX6; GPx-7; GSHPx-7
UniProt Entry Name
GPX7_HUMAN
Uniprot Description
GPX7: Belongs to the glutathione peroxidase family.
Protein type: Secreted; EC 1.11.1.9; Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Oxidoreductase
Chromosomal Location of Human Ortholog: 1p32
Cellular Component: endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region
Molecular Function: peroxidase activity; glutathione peroxidase activity
Biological Process: response to reactive oxygen species
Research Articles on GPX7
Similar Products
Product Notes
The GPX7 gpx7 (Catalog #AAA8114982) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 408aa. AAA Biotech's GPX7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GPX7 gpx7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu18 7. It is sometimes possible for the material contained within the vial of "GPX7, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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