GHRH cell lysate
Human GHRH HEK293 Overexpression Lysate
Gene Names
GHRH; GRF; INN; GHRF; MGC119781; GHRH
Applications
Western Blot
Synonyms
GHRH; Human GHRH HEK293 Overexpression Lysate; Human GHRH HEK293 Cell Lysate (WB positive control); growth hormone releasing hormone; Human GHRF Overexpression Lysate; Human GRF Overexpression Lysate; Human INN Overexpression Lysate; GHRH cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
304aa
Sequence
Tyr32-Leu75
Applicable Applications for GHRH cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human GHRH (NP_066567.1) (Tyr32-Leu75) was expressed with the Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GHRH cell lysate
The role of hypothalamic growth hormone-releasing hormone (GHRH) in the release of growth hormone (GH) from the pituitary is well established. Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in infertility. Growth hormone releasing hormone (GHRH) has recently been shown to increase the level of gamma-aminobutyric acid (GABA) and activate GABA receptors (GABARs) in the cerebral cortex. GABA is an inhibitory neurotransmitter that can inhibit seizures. GHRH may play an important role in inhibiting seizures by activating GABAARs. GHRH is produced by tumor cells, acts in an autocrine/paracrine manner, and requires the presence of GHRH receptor (GHRH-R) on the tumor cells to exert its effects. GHRH activity can be effectively blocked by synthetic antagonists of its receptor and hence, the expression of GHRH-R by tumor cells could serve as a predictor of response to GHRH-R antagonist therapy. The neurovascular protective effect of GHRH analogs during the early stage of diabetic retinopathy through their antioxidant and anti-inflammatory properties. GHRH antagonists can be a therapeutic option for thyroid cancer patients.
This Human GHRH overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GHRH protein from the overexpression lysate was verified.
This Human GHRH overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of GHRH protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
somatoliberin isoform 1 preproprotein [Homo sapiens]
NCBI Official Synonym Full Names
growth hormone releasing hormone
NCBI Official Symbol
GHRH
NCBI Official Synonym Symbols
GRF; INN; GHRF; MGC119781; GHRH
NCBI Protein Information
somatoliberin; sermorelin; somatorelin; somatocrinin; OTTHUMP00000030915; growth hormone-releasing factor; growth hormone-releasing hormone
UniProt Protein Name
Somatoliberin
Protein Family
UniProt Gene Name
GHRH
UniProt Synonym Gene Names
GHRF; GRF; GHRH
UniProt Entry Name
SLIB_HUMAN
Uniprot Description
GHRH: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: extracellular space; extracellular region; terminal button
Molecular Function: growth hormone-releasing hormone activity; growth hormone-releasing hormone receptor binding
Biological Process: response to food; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of growth hormone secretion; growth hormone secretion; cAMP-mediated signaling; positive regulation of circadian sleep/wake cycle, REM sleep; cell-cell signaling; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; adenohypophysis development; positive regulation of multicellular organism growth; G-protein signaling, adenylate cyclase activating pathway
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Product Notes
The GHRH ghrh (Catalog #AAA8115517) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 304aa. AAA Biotech's GHRH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GHRH ghrh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Tyr32-Leu7 5. It is sometimes possible for the material contained within the vial of "GHRH, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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