beta Defensin 1/DEFB1 Cell Lysate | DEFB1 cell lysate
Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Applications
Western Blot
Synonyms
beta Defensin 1/DEFB1; Human beta Defensin 1/DEFB1 HEK293 Overexpression Lysate; Human DEFB1/Beta-defensin 1 HEK293 Cell Lysate (WB positive control); defensin; beta 1; Human BD1 Overexpression Lysate; Human DEFB-1 Overexpression Lysate; Human DEFB101 Overexpression Lysate; Human HBD1 Overexpression Lysate; DEFB1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
285aa
Sequence
Met1-Lys68
Applicable Applications for DEFB1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human DEFB1 (NP_005209.1) (Met1-Lys68) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for DEFB1 cell lysate
The DEFB1 gene, encoding for the constitutively expressed human beta-defensin 1 (hBD1) antimicrobial peptide is a potential candidate when studying genetic susceptibility to caries. DEFB1 genetic variations have been reported as contributing to hBD1 production impairment, leading to a greater susceptibility to be infected by oral pathogens, also leading to periodontitis. To counteract host immunity, Cryptosporidium parvum has evolved multiple strategies to suppress host antimicrobial defense. One such strategy is to reduce the production of the antimicrobial peptide beta-defensin 1 (DEFB1) by host epithelial cells. Beta-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity.
This Human beta Defensin 1/DEFB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of beta Defensin 1/DEFB1 protein from the overexpression lysate was verified.
This Human beta Defensin 1/DEFB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of beta Defensin 1/DEFB1 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,420 Da
NCBI Official Full Name
beta-defensin 1 preproprotein
NCBI Official Synonym Full Names
defensin, beta 1
NCBI Official Symbol
DEFB1
NCBI Official Synonym Symbols
BD1; HBD1; DEFB-1; DEFB101
NCBI Protein Information
beta-defensin 1; BD-1; beta defensin 1; beta-defensin-1
UniProt Protein Name
Beta-defensin 1
Protein Family
UniProt Gene Name
DEFB1
UniProt Synonym Gene Names
BD1; HBD1; BD-1; hBD-1
UniProt Entry Name
DEFB1_HUMAN
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]
Uniprot Description
defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular space; Golgi lumen; extracellular region
Biological Process: G-protein coupled receptor protein signaling pathway; defense response to Gram-positive bacterium; innate immune response in mucosa; response to bacterium; antibacterial humoral response; innate immune response; immune response; response to testosterone stimulus; chemotaxis; acute inflammatory response
Research Articles on DEFB1
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Product Notes
The DEFB1 defb1 (Catalog #AAA8115457) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 285aa. AAA Biotech's beta Defensin 1/DEFB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the DEFB1 defb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Lys68. It is sometimes possible for the material contained within the vial of "beta Defensin 1/DEFB1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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