Fibroblast growth factor 21 recombinant protein

Human fibroblast growth factor 21

Cat. Num. AAA400127

• Purity: Greater than 98.0% as determined by:; (a) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 mM NaCl, pH 8.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Synonyms: Fibroblast growth factor 21; Human fibroblast growth factor 21; Fibroblast growth factor 21 recombinant protein

• Host: E. coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 mM NaCl, pH 8.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Form/Format: The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3 previously adjusted pH 8.
• Sequence Length: 209

• Physical Appearance: White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized human FGF-21 in 0.4% NaHCO3 or water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
• Amino Acid Sequence: The sequence of the first eight N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro-Asp-Ser-Ser.
• Dimers and Aggregates: Less than 2% as determined by gel filtration chromatography.
• Endotoxin: Less than 0.05 ng/µg (0.5 EU/µg) of hFGF-21.
• Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences
• Preparation and Storage: Lyophilized hFGF-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization hFGF-21 can be stored at 4C, pH 8 for up to 4 weeks. For long term storage and more diluted solutions (but not for in vivo experiments) it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for Fibroblast growth factor 21 recombinant protein

Human FGF-21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Recombinant human FGF-21 is a 19.5 kDa protein containing 182 amino acid residues. Human FGF-21 is purified by proprietary ion exchange chromatographic techniques.

Product Categories/Family for Fibroblast growth factor 21 recombinant protein

Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 9506597
• NCBI GeneID: 26291
• NCBI Accession #: NP_061986.1
• NCBI GenBank Nucleotide #: NM_019113.2
• Molecular Weight: 22,300 Da
• NCBI Official Full Name: fibroblast growth factor 21
• NCBI Official Synonym Full Names: fibroblast growth factor 21
• NCBI Official Symbol: FGF21
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF21
• UniProt Synonym Gene Names: FGF-21
• UniProt Entry Name: FGF21_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell-cell signaling; positive regulation of cell proliferation; positive regulation of glucose import; positive regulation of low-density lipoprotein receptor biosynthetic process; response to activity; response to nutrient levels; signal transduction; unfolded protein response