Fibroblast Growth Factor-19 Recombinant Protein | FGF 19 recombinant protein

Recombinant Human Fibroblast Growth Factor-19 His Tag

Cat. Num. AAA142156

• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE.
• Synonyms: Fibroblast Growth Factor-19; Recombinant Human Fibroblast Growth Factor-19 His Tag; FGF 19 Human; His; Fibroblast Growth Factor-19 Human Recombinant; His Tag; Fibroblast growth factor 19; FGF-19; FGF 19 His; FGF 19 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE.
• Form/Format: Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5.; Filtered white lyophilized powder.
• Sequence: MRGSHHHHHH GMASLAFSDA GPHVHYGWGD PIRLRHLYTS GPHGLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK
• Sequence Length: 216

• Solubility: Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for FGF 19 recombinant protein

Description: Fibroblast Growth Factor -19 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23 kDa. The amino acid sequence of the recombinant human FGF19 is 100% homologous to the amino acid sequence of the human FGF19 without signal sequence and contains his tag at N-terminal The FGF-19 is purified by proprietary chromatographic techniques.

Introduction: The FGFs are a family of more than 20 small (~1726 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response.FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-19, has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.

Product Categories/Family for FGF 19 recombinant protein

Cytokines and Growth Factors; Growth Factors; Fibroblast Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 4826726
• NCBI GeneID: 9965
• NCBI Accession #: NP_005108.1
• NCBI GenBank Nucleotide #: NM_005117.2
• UniProt Accession #: O95750
• Molecular Weight: 24,003 Da
• NCBI Official Full Name: fibroblast growth factor 19
• NCBI Official Synonym Full Names: fibroblast growth factor 19
• NCBI Official Symbol: FGF19
• NCBI Protein Information: fibroblast growth factor 19; FGF-19
• UniProt Protein Name: Fibroblast growth factor 19
• UniProt Gene Name: FGF19
• UniProt Synonym Gene Names: FGF-19
• UniProt Entry Name: FGF19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; heart development; positive regulation of JNK cascade; positive regulation of glucose import; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of protein amino acid phosphorylation; neural crest cell migration