HotStart Taq DNA Polymerase

HotStart Taq DNA Polymerase (Mg Plus Buffer)

Cat. Num. AAA9141664

• Applications: Multiplex PCR, Specialty PCR, PCR, Routine PCR
• Synonyms: HotStart Taq DNA Polymerase; HotStart Taq DNA Polymerase (Mg Plus Buffer); HotStart Taq DNA Polymerase dna polymerase

• Host: E Coli
• Form/Format: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 at 25 degree C
• Concentration: 5,000 U/ml (varies by lot)

• Applicable Applications for HotStart Taq DNA Polymerase dna polymerase: Multiplex PCR, Specialty PCR, PCR, Routine PCR
• Source Note: An E Coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticu YT-1.
• Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75 degree C.
• Reaction Conditions: 1X PCR Reaction Buffer, Mg2+ plus
• Reaction Buffer or Mix: 1X PCR Reaction Buffer, Mg2+ plus:; 20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, pH 8.8 at 25 degree C
• Heat Inactivation: No
• 5' - 3' Exonuclease: Yes
• 3' - 5' Exonuclease: No
• Strand Displacement: +
• Resulting Ends: Single-base 3' Overhangs
• Error Rate: ~ 285x10^6 bases
• Preparation and Storage: Store at -20 degree C.

Related Product Information for HotStart Taq DNA Polymerase dna polymerase

HotStart Taq DNA polymerase is an innovative antibody-modified thermostable enzyme. The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification. When heated to 95 degree C, the antibody bound to the enzyme was rapidly inactivated and did not affect the subsequent Taq DNA polymerase reaction. The enzyme has the advantages of good specificity, high sensitivity, good reproducibility and high amplification efficiency.