LEDGF Human Active Protein
LEDGF, Recombinant Human (Lens Epithelium-derived Growth Factor, Transcriptional Coactivator p75/p52, PSIP1, PC4 and SFRS1-interacting Protein, PSIP2, DFS70, Dense Fine Speckles 70kD Protein, PAIP, CLL-associated Antigen KW-7)
Purity
Highly Purified~95% (SDS-PAGE, reducing conditions)
Synonyms
LEDGF Human; LEDGF; Recombinant Human (Lens Epithelium-derived Growth Factor; Transcriptional Coactivator p75/p52; PSIP1; PC4 and SFRS1-interacting Protein; PSIP2; DFS70; Dense Fine Speckles 70kD Protein; PAIP; CLL-associated Antigen KW-7); LEDGF Human active protein
Host
E Coli-derived, Met1-Gln333.
Purity/Purification
Highly Purified
~95% (SDS-PAGE, reducing conditions)
~95% (SDS-PAGE, reducing conditions)
Form/Format
Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.
Application Notes
SDS-PAGE: 52kD, reducing conditions
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 1.5-15ug/ml on a nitrocellulose-coated microplate.
Endotoxin Level
1EU/1ug (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for LEDGF Human active protein
Lens epithelium-derived growth factor (LEDGF; also PC4 and SFRS1-interacting protein, PSIP, transcriptional co-activator p75/p52, and dense fine speckles 70kD protein) is a ubiquitously expressed, 75kD member of the hepatoma-derived growth factor (HDGF) family of proteins. Human LEDGF is 530aa in length. LEDGF contains a PWWP domain (aa 1-64), a nuclear localization signal (aa146-156), two coiled-coil regions (aa306-334 and 371-395), and four helical regions (aa347-362, 370-381, 394-403 and 410-425). In addition, there are multiple serine and threonine residues that are sites of potential phosphorylation. The protein is highly charged, with lysine, arginine, glutamate, and aspartate comprising 39% of the total residues. Two splicing variants produce a second isoform known as p52. p52 has an 8aa substitution corresponding to aa326-333 of LEDGF, and a deletion of the final 197aa found in LEDGF. Human LEDGF shares 92% aa sequence identity with mouse and rat LEDGF. LEDGF functions as a transcriptional co-activator that is involved in neuroepithelial stem cell differentiation and neurogenesis. It is also a survival factor that is inducible by oxidative stress and protects cells from various stresses by upregulating stress- responsive genes. For example, TNF-alpha elevates the expression of LEDGF, which increases the expression of endogenous gamma-GHS-HS, the catalytic subunit of the regulating enzyme in GSH biosynthesis that constitutes a protective mechanism in limiting oxidative stress induced by inflammatory cytokines. LEDGF is also a major autoantigen in atopic dermatitis and other inflammatory conditions involving dysregulated apoptosis. Anti-LEDGF autoantibodies have been shown to have cytotoxic activity, suggesting their involvement in pathogenesis. In apoptotic cells, caspases cleave this protein at three sites within functionally important domains, generating two fragments of 65 and 58kD. Caspase cleavage abolishes the survival function of LEDGF and may generate variants of the protein that enhance apoptosis. LEDGF has also been shown to interact with lentiviral integrase (IN) proteins, including HIV-1 IN, directly, determining their nuclear localization and their tight association with nuclear DNA. This prevents proteosomal degradation of these proteins.
Product Categories/Family for LEDGF Human active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LEDGF Human (Catalog #AAA637571) is an Active Protein produced from E Coli-derived, Met1-Gln333. and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 52kD, reducing conditions. Researchers should empirically determine the suitability of the LEDGF Human for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEDGF Human, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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