Calcineurin Active Protein | PPP3CA active protein

Calcineurin (human), (recombinant)

Cat. Num. AAA566577

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• Purity: >=90% (SDS-PAGE)
• Synonyms: Calcineurin; Calcineurin (human); (recombinant); PPP3CA active protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE)
• Form/Format: Liquid. In 1X KI-128 Assay Buffer with increased DTT concentration (50mM TRIS, pH7.5, containing 100mM sodium chloride, 6mM MgCl2, 5mM DTT, 0.025% NP-40 and 0.5mM CaCl2)
• Concentration: 100U/ul (varies by lot)

• Source Note: Human calcineurin Aalpha (MW 60kDa) and calcineurin B (MW 15kDa) heterodimer. Both subunits are coexpressed in a construct with yeast myristoyl-CoA:protein N-myristoyltransferase. The resulting highly active calcineurin (protein phosphatase 2B) is N-myristoylated on the CaNB subunit, similar to the native protein
• Specific Activity: One unit= 1pmol/min using 0.15mM RII phosphopeptide substrate in the presence of calcium/calmodulin at 30 degree C. In the absence of calmodulin, the activity is <1%
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Product Categories/Family for PPP3CA active protein

Proteostasis; Posttranslational Modifications

NCBI and Uniprot Product Information

• NCBI GI #: 1352673
• NCBI GeneID: 5530
• UniProt Accession #: Q08209; P63098
• Molecular Weight: 521
• NCBI Official Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatas
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Calcineurin
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle