Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate Culture Media
Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)
Purity
Cell Culture Grade
Synonyms
Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine; Zinc Sulfate; Zinc Sulfate (Powder); Zinc Sulfate culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light pink, homogenous, free flowing powder
Solubility
Light pink, clear, complete
pH
7.3
Endotoxin
≤ 1Eu/ml
Directions per Liter
Dissolve 11.75g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/liter
Inorganic salts:
Calcium Chloride•2H2O: 0.165
Cupric Sulfate•5H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride•6H2O: 0.09963
Magnesium Sulfate: 0.02528
Potassium Chloride: 0.305
Potassium Phosphate Mono: 0.06124
Sodium Chloride: 7.517
Sodium Phosphate Dibasic: 0.1324
Zinc Sulfate•7H2O: 0.000863
Amino Acids:
L-Alanine: 0.018
L-Arginine•HCl: 0.422
L-Asparagine•H2O: 0.03
L-Aspartic Acid: 0.026
L-Cysteine•HCL•H2O: 0.07026
L-Glutamic Acid: 0.03
L-Glutamine: 0.292
Glycine: 0.016
Calcium Chloride•2H2O: 0.165
Cupric Sulfate•5H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride•6H2O: 0.09963
Magnesium Sulfate: 0.02528
Potassium Chloride: 0.305
Potassium Phosphate Mono: 0.06124
Sodium Chloride: 7.517
Sodium Phosphate Dibasic: 0.1324
Zinc Sulfate•7H2O: 0.000863
Amino Acids:
L-Alanine: 0.018
L-Arginine•HCl: 0.422
L-Asparagine•H2O: 0.03
L-Aspartic Acid: 0.026
L-Cysteine•HCL•H2O: 0.07026
L-Glutamic Acid: 0.03
L-Glutamine: 0.292
Glycine: 0.016
Powder
L-Histidine•HCl•H2O: 0.042
L-Isoleucine: 0.0078
L-Leucine: 0.0262
L-Lycine•HCl: 0.073
L-Methionine: 0.009
L-Phenylalanine: 0.01
L-Proline: 0.07
L-Serine: 0.021
L-Threonine: 0.0238
L-Tryptophan: 0.004
L-Tyrosine•2Na•2H2O: 0.01586
L-Valine: 0.0234
Vitamins:
L-Ascorbic Acid•Na: 0.15
D-Biotin: 0.0000073
Choline Chloride: 0.01396
Vitamin B-12: 0.00136
Folic Acid: 0.00132
myo-Inositol: 0.01802
Niacinamide: 0.00004
D-Pantothenic Acid, Ca: 0.000238
Pyridoxine•HCl: 0.00006
Riboflavin: 0.00004
Thiamine•HCl: 0.000337
Other:
Thymidine: 0.0007
D-Glucose: 1.802
Hypoxanthine: 0.00404
Linoleic Acid: 0.00009
Thioctic Acid: 0.000206
Phenol Red, Sodium: 0.00125
Putrescine•2HCl: 0.000161
Pyruvic Acid, Sodium: 0.22
Total: 11.75 g/liter
L-Isoleucine: 0.0078
L-Leucine: 0.0262
L-Lycine•HCl: 0.073
L-Methionine: 0.009
L-Phenylalanine: 0.01
L-Proline: 0.07
L-Serine: 0.021
L-Threonine: 0.0238
L-Tryptophan: 0.004
L-Tyrosine•2Na•2H2O: 0.01586
L-Valine: 0.0234
Vitamins:
L-Ascorbic Acid•Na: 0.15
D-Biotin: 0.0000073
Choline Chloride: 0.01396
Vitamin B-12: 0.00136
Folic Acid: 0.00132
myo-Inositol: 0.01802
Niacinamide: 0.00004
D-Pantothenic Acid, Ca: 0.000238
Pyridoxine•HCl: 0.00006
Riboflavin: 0.00004
Thiamine•HCl: 0.000337
Other:
Thymidine: 0.0007
D-Glucose: 1.802
Hypoxanthine: 0.00404
Linoleic Acid: 0.00009
Thioctic Acid: 0.000206
Phenol Red, Sodium: 0.00125
Putrescine•2HCl: 0.000161
Pyruvic Acid, Sodium: 0.22
Total: 11.75 g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate culture media
Ham's Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured.
Ham's F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues.
Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.
Ham's F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues.
Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.
Product Categories/Family for Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate culture media
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Product Notes
The Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Catalog #AAA652750) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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