Dulbecco's MEM Culture Media

Dulbecco's MEM (DMEM) Ham's F-12 w/HEPES, NaHCO3, w/o Amino Acids (Powder)

Cat. Num. AAA653351

• Purity: Cell Culture Grade
• Synonyms: Dulbecco's MEM; Dulbecco's MEM (DMEM) Ham's F-12 w/HEPES; NaHCO3; w/o Amino Acids (Powder); Dulbecco's MEM culture media

• Purity/Purification: Cell Culture Grade

• Endotoxin Level: ≤ 1EU/ml
• Solubility: Orange, clear, complete.
• Appearance: Light orange, homogenous, free flowing powder.
• pH: 6.8
• Directions per Liter: Dissolve 15.68g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
• Components shown as mg/liter: Inorganic Salts:; Calcium Chloride•2H20 - 154.520; Cupric Sulfate•5H2O - 0.0013; Ferric Nitrate•9H2O - 0.050; Ferrous Sulfate•7H2O - 0.420; Magnesium Chloride - 28.570; Magnesium Sulfate - 48.830; Potassium Chloride - 311.830; Sodium Bicarbonate - 1200.000; Sodium Chloride - 6999.500; Sodium Phosphate Dibasic - 71.060; Sodium Phosphate Monobasic - 54.350; Zinc Sulfate•7H2O - 0.432; ; Amino Acids; L-Alanine -; L-Arginine -; L-Asparagine•H2O -; L-Aspartic Acid -; L-Cysteine•HCI•H2O -; L-Cystine•2HCI -; L-Glutamic Acid -; L-Glutamine -; Glycine -; L-Histidine HCl•H2O -; L-Isoleucine -; L-Leucine -; L-Lysine•HCI -; L-Methionine -; L-Phenylalanine -; L-Proline -; L-Serine -; L-Threonine -; L-Tryptophan -; L-Tyrosine•2Na•2H20 -; L-Valine -; ; Vitamins; Biotin - 0.004; Choline Chloride - 8.980; Vitamin B12 - 0.679; Folic Acid - 2.660; i-Inositol - 0.013; Nicotinamide - 2.018; D-Pantothenic Acid•Ca - 2.120; Pyridoxal HCI - 2.000; Pyridoxine HCI 0.031; Riboflavin - 0.219; Thiamine HCI - 2.170; ; Other; Thymidine - 0.365; D-Glucose - 3150.000; HEPES Free Acid - 3574.000; Hypoxanthine, Sodium - 2.100; Linoleic Acid - 0.042; Lipoic Acid - 0.105; Phenol Red - 8.305; Putrescine•2HCl - 0.081; Sodium Pyruvate - 55.050; Total: 15.68g/L
• Preparation and Storage: Store powdered media at RT.; Opened bottles should be capped tightly and kept in a dark, low humidity environment.; Prepared media should be kept at 4°C and used within a short period of time.

Related Product Information for Dulbecco's MEM culture media

Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM "High Glucose" with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham's mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham's F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.

Product Categories/Family for Dulbecco's MEM culture media

Culture Media; Media-Cell Culture; DMEM