MAP4K1 blocking peptide

Phospho-MAP4K1 (Y381) Blocking Peptide

• Gene Names: MAP4K1; HPK1
• Synonyms: MAP4K1; Phospho-MAP4K1 (Y381) Blocking Peptide; Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; HPK1; MAP4K1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 374-388 of HUMAN MAP4K1
• Form/Format: Lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 821

• Reconstitution: Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAP4K1 blocking peptide

The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 110611908
• NCBI GeneID: 11184
• NCBI Accession #: NP_001036065.1; NP_009112.1
• NCBI GenBank Nucleotide #: NM_001042600.3
• UniProt Accession #: Q92918
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 1
• NCBI Official Symbol: MAP4K1
• NCBI Official Synonym Symbols: HPK1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 1
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K1
• UniProt Synonym Gene Names: HPK1; MEK kinase kinase 1; MEKKK 1
• UniProt Entry Name: M4K1_HUMAN

Uniprot Description

HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.

Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.4

Cellular Component: membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; activation of MAPKKK activity; response to stress; protein amino acid phosphorylation; activation of JNK activity