MAK blocking peptide

MAK Antibody (C-term) Blocking Peptide

• Gene Names: MAK; RP62
• Synonyms: MAK; MAK Antibody (C-term) Blocking Peptide; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase; MAK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human MAK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 623

• Cellular Location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment. Note: Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
• Tissue Location: Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAK blocking peptide

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 13432166
• NCBI GeneID: 4117
• UniProt Accession #: P20794; Q547D0; Q9NUH7; F1T0K6; G1FL29
• Molecular Weight: 66,345 Da
• NCBI Official Full Name: Serine/threonine-protein kinase MAK
• NCBI Official Synonym Full Names: male germ cell associated kinase
• NCBI Official Symbol: MAK
• NCBI Official Synonym Symbols: RP62
• NCBI Protein Information: serine/threonine-protein kinase MAK
• UniProt Protein Name: Serine/threonine-protein kinase MAK
• Protein Family: Makatoxin
• UniProt Gene Name: MAK
• UniProt Entry Name: MAK_HUMAN

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Nuclear receptor co-regulator; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: centrosome; midbody; nucleus; photoreceptor inner segment; photoreceptor outer segment

Molecular Function: protein binding; transcription coactivator activity

Biological Process: photoreceptor cell maintenance; protein amino acid autophosphorylation; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 62