HGK blocking peptide

HGK (R430) Peptide

Cat. Num. AAA3002351

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Applications: Blocking
• Purity: Synthetic peptide HGK (R430). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Synonyms: HGK; HGK (R430) Peptide; Mitogen-activated protein kinase kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; HPK/GCK-like kinase HGK; Nck interacting kinase; MEKKK 4; MAP4K4; NIK; KIAA0687; HGK blocking peptide

• Specificity: This peptide can be used with studies using MBS3002350 HGK (R430) pAb.
• Purity/Purification: Synthetic peptide HGK (R430). (Note: the amino acid sequence is proprietary).; The purity is >98%.
• Form/Format: 1 mg/ml in DI water.
• Sequence Length: 1239

• Applicable Applications for HGK blocking peptide: Blocking
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for HGK blocking peptide

Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include NIK, KHS, GLK, YSK1, HPK1, Krs-1, Krs-2, and GC kinase. NIK (Nck interacting kinase), like many of the STE20-like kinases, has been shown to activate the SAPK/JNK stress response pathway. Both the kinase domain and the C-terminal regulatory domain of NIK are required for full activation. NIK interacts with MEKK1 and is thought to act upstream of MEKK1 in the SAPK/JNK signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 336020358
• NCBI GeneID: 9448
• NCBI Accession #: NP_001229488.1
• NCBI GenBank Nucleotide #: NP_001229488.1
• UniProt Accession #: O95819
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; regulation of catalytic activity; regulation of JNK cascade; protein amino acid phosphorylation