CCL26/Eotaxin-3 Cell Lysate | CCL26 cell lysate
Human CCL26/Eotaxin-3 Baculovirus-Insect Overexpression Lysate
Gene Names
CCL26; IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha
Applications
Western Blot
Synonyms
CCL26/Eotaxin-3; Human CCL26/Eotaxin-3 Baculovirus-Insect Overexpression Lysate; Human CCL26/Eotaxin-3 Insect Cell Lysate (WB positive control); chemokine (C-C motif) ligand 26; Human IMAC Overexpression Lysate; Human MIP-4a Overexpression Lysate; Human MIP-4alpha Overexpression Lysate; Human SCYA26 Overexpression Lysate; Human TSC-1 Overexpression Lysate; CCL26 cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
71aa
Sequence
Met1-Leu94
Applicable Applications for CCL26 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Sequence Construction
A DNA sequence encoding the human CCL26 (NP_006063.1) (Met1-Leu94) was expressed.
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CCL26 cell lysate
The eotaxin subfamily of CC chemokines consists of eotaxin-1/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26. All eotaxins induce the trafficking of eosinophils to the sites of inflammation via CC chemokine receptor 3 (CCR3), which is also expressed by several different cell types, including basophils, dendritic cells, smooth muscle cells, epithelial cells and fibroblasts. The sequence similarity between the three eotaxins is limited (<4%), but their functional properties are very similar. Eotaxin-1 and -2 are expressed by both haematopoietic and non-haematopoietic cells, but eotaxin-3 expression has been reported to be limited to non-haematopoietic cells. Interleukin (IL)-4 is the main inducer for eotaxin-3 expression, whereas eotaxin-1 is up-regulated by IL-4 and the proinflammatory cytokine tumour necrosis factor (TNF)-alpha. Eotaxin-3 is expressed in vascular endothelial cells and human dermal fibroblasts after IL-4 and IL-13 stimulation, and this is dependent upon the IL-4-/IL-13-specific transcription factor, signal transducers and activator of transcription (STAT)-6. Eotaxin-3 is expressed on the surface of IL-4-stimulated endothelial cells and promotes eosinophil transmigration.
This Human CCL26/Eotaxin-3 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CCL26/Eotaxin-3 protein from the overexpression lysate was verified.
This Human CCL26/Eotaxin-3 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CCL26/Eotaxin-3 protein from the overexpression lysate was verified.
References
Takahashi K, Imaeda H, Fujimoto T, et al. Regulation of eotaxin-3/CC chemokine ligand 26 expression by T helper type 2 cytokines in human colonic myofibroblasts. Clinical and Experimental Immunology. 2013;173(2):323-331.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,648 Da
NCBI Official Full Name
C-C motif chemokine 26
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 26
NCBI Official Symbol
CCL26
NCBI Official Synonym Symbols
IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha
NCBI Protein Information
C-C motif chemokine 26; CC chemokine IMAC; MIP-4-alpha; chemokine N1; eotaxin-3; macrophage inflammatory protein 4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; small-inducible cytokine A26; thymic stroma
UniProt Protein Name
C-C motif chemokine 26
Protein Family
UniProt Gene Name
CCL26
UniProt Synonym Gene Names
SCYA26; MIP-4-alpha; TSC-1
UniProt Entry Name
CCL26_HUMAN
NCBI Description
This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
CCL26: Chemotactic for eosinophils and basophils. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: extracellular space
Molecular Function: chemokine activity
Biological Process: cell-cell signaling; positive regulation of actin filament polymerization; immune response; positive regulation of endothelial cell proliferation; chemotaxis; inflammatory response; signal transduction; positive regulation of cell migration
Research Articles on CCL26
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Product Notes
The CCL26 ccl26 (Catalog #AAA8113976) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 71aa. AAA Biotech's CCL26/Eotaxin-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CCL26 ccl26 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu94. It is sometimes possible for the material contained within the vial of "CCL26/Eotaxin-3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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