Aminopeptidase, Aeromonas Active Protein
Aminopeptidase, Aeromonas, Recombinant (AAP)
Applications
Western Blot, Immunocytochemistry
Purity
98% as determined by RP-HPLC and analysis by reducing and non-reducing SDS-PAGE stained Coomassie blue gel.
Synonyms
Aminopeptidase; Aeromonas; Recombinant (AAP); Aeromonas active protein
Host
Aeromonas proteolytica
Purity/Purification
98% as determined by RP-HPLC and analysis by reducing and non-reducing SDS-PAGE stained Coomassie blue gel.
Form/Format
Sterile filtered liquid in 10mM Tris-HCl, 100mM, sodium chloride, 5uM zinc sulfate, pH 8.0.
Applicable Applications for Aminopeptidase, Aeromonas active protein
Western Blot (WB), Immunocytochemistry (ICC)
Activity
~120 Units/mg protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Aminopeptidase, Aeromonas active protein
The Enzyme (29kD) Aeromonas Aminopeptidase is used in the processing of pharmaceutical proteins produces by genetic engineering as well as for physical and structural investigations and for sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at -X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Leucine aminopeptidase from Aeromonas proteolytica (AAP) are widely distributed in bacteria, yeast, plant, and animal tissues and, therefore, have a wide variety of biological functions that include protein maturation, protein degradation, hormone level regulation, and cell-cycle control. The importance of understanding the mechanism of action of aminopeptidases is underscored by the recent observation that the naturally occurring peptide analog inhibitor, bestatin, was shown to significantly decrease HIV viral in humans by inhibiting leucine aminopeptidase activity. Another physiologic role for aminopeptidases involves angiogenesis, the formation of new blood vessels, which is essential for tumor growth. Recently, a type-2 methionyl aminopeptidase from eukaryotes was identified as the molecular target for the anti-angiogenesis drugs ovalicin and fumagillin, one of which is in phase III clinical trials. Thus, the inhibition of aminopeptidase activity in viruses and malignant tumors is critically important in preventing the growth and proliferation of these types of cells.
Leucine aminopeptidase from Aeromonas proteolytica (AAP) are widely distributed in bacteria, yeast, plant, and animal tissues and, therefore, have a wide variety of biological functions that include protein maturation, protein degradation, hormone level regulation, and cell-cycle control. The importance of understanding the mechanism of action of aminopeptidases is underscored by the recent observation that the naturally occurring peptide analog inhibitor, bestatin, was shown to significantly decrease HIV viral in humans by inhibiting leucine aminopeptidase activity. Another physiologic role for aminopeptidases involves angiogenesis, the formation of new blood vessels, which is essential for tumor growth. Recently, a type-2 methionyl aminopeptidase from eukaryotes was identified as the molecular target for the anti-angiogenesis drugs ovalicin and fumagillin, one of which is in phase III clinical trials. Thus, the inhibition of aminopeptidase activity in viruses and malignant tumors is critically important in preventing the growth and proliferation of these types of cells.
Product Categories/Family for Aminopeptidase, Aeromonas active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29kD
NCBI Official Full Name
aminopeptidase
Similar Products
Product Notes
The Aminopeptidase, Aeromonas (Catalog #AAA637275) is an Active Protein produced from Aeromonas proteolytica and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aminopeptidase, Aeromonas can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the Aminopeptidase, Aeromonas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aminopeptidase, Aeromonas, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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