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Adenosine Deaminase, tRNA-specific 1 Recombinant Protein | ADAT1 recombinant protein

Adenosine Deaminase, tRNA-specific 1 (ADAT1) Recombinant, Human

Gene Names
ADAT1; HADAT1
Purity
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
Adenosine Deaminase; tRNA-specific 1; tRNA-specific 1 (ADAT1) Recombinant; Human; ADAT1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% sodium azide.
Application Notes
ELISA, MS, Inhibition Assays, Western Blotting
Preparation and Storage
-20 degree C
Stability: Recombinant Human ADAT-1although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for ADAT1 recombinant protein
This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA.

Recombinant Human ADAT-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-502 and having a total molecular mass of 57.7kD. ADAT-1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
Product Categories/Family for ADAT1 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
57.7kD
NCBI Official Full Name
Adenosine deaminase, tRNA-specific 1
NCBI Official Synonym Full Names
adenosine deaminase, tRNA-specific 1
NCBI Official Symbol
ADAT1
NCBI Official Synonym Symbols
HADAT1
NCBI Protein Information
tRNA-specific adenosine deaminase 1; adenosine deaminase acting on tRNA; tRNA-specific adenosine-37 deaminase
UniProt Protein Name
tRNA-specific adenosine deaminase 1
UniProt Gene Name
ADAT1
UniProt Synonym Gene Names
hADAT1
UniProt Entry Name
ADAT1_HUMAN

NCBI Description

This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAT1: Specifically deaminates adenosine-37 to inosine in tRNA- Ala. Belongs to the ADAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.4.34; RNA processing; Hydrolase

Chromosomal Location of Human Ortholog: 16q23.1

Molecular Function: tRNA-specific adenosine deaminase activity; RNA binding; metal ion binding

Biological Process: RNA processing; tRNA processing

Research Articles on ADAT1

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Product Notes

The ADAT1 adat1 (Catalog #AAA635560) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. ELISA, MS, Inhibition Assays, Western Blotting. Researchers should empirically determine the suitability of the ADAT1 adat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenosine Deaminase, tRNA-specific 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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