Beta-1,4-Galactosyltransferase 1 Active Protein | B4GALT1 active protein

Beta-1,4-Galactosyltransferase 1, Recombinant, Human (B4GalT1)

Cat. Num. AAA638056

• Gene Names: B4GALT1; GGTB2
• Purity: Highly Purified; 95% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Beta-1; 4-Galactosyltransferase 1; Recombinant; Human (B4GalT1); B4GALT1 active protein

• Purity/Purification: Highly Purified; 95% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a liquid in 25mM Tris, 150mM sodium chloride, pH 7.5.

• Application Notes: SDS-PAGE: 45 55kD
• Immunogen: NS0-derived recombinant human B4GalT1, NP_001488.
• Activity: Measured by its ability to transfer galactose from UDP Galactose to N-Acetyl-alpha-D-glucosamine. The specific activity is >2000pmol/min/ug, as measured under the described conditions. See protocol.
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for B4GALT1 active protein

Beta-1,4-Galactosyltransferase 1 (B4GalT1) is one of seven beta1, 4 galactosyltransferases that transfer galactose in a beta1,4 linkage to acceptor sugars including Gl cNAc, and Gl c, and Xyl. By sequence similarity, the B4GalTs form four groups: B4GalT1 and B4GalT2, B4GalT3 and B4GalT4, B4GalT5 and B4GalT6 and B4GalT7. B4GalT1 is unique among the seven enzymes because it can be expressed either as membrane associated form or secreted form. The secreted form is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-l actalbumin to catalyze the synthesis of lactose. The membrane form can reside either in the Golgi apparatus, where it adds galactose to N-acetylglucosamine residues, or on cell surface, where it functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. The two enzymatic forms result from alternate transcription initiation sites and post­translational processing. Defects in B4GalT1 are the cause of congenital disorder of glycosylation type 2D(CDG2D).

Product Categories/Family for B4GALT1 active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 29135321
• NCBI GeneID: 281781
• NCBI Accession #: NP_803478.1
• NCBI GenBank Nucleotide #: NM_177512.2
• UniProt Accession #: P08037; Q0VC05; Q8MIG0
• Molecular Weight: 40kD
• NCBI Official Full Name: beta-1,4-galactosyltransferase 1
• NCBI Official Synonym Full Names: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
• NCBI Official Symbol: B4GALT1
• NCBI Official Synonym Symbols: GGTB2
• NCBI Protein Information: beta-1,4-galactosyltransferase 1; b4Gal-T1; beta4Gal-T1; beta-1,4-GalTase 1; beta-galactosyltransferase 2 (glycoprotein); glycoprotein-4-beta-galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
• UniProt Protein Name: Beta-1,4-galactosyltransferase 1
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT1
• UniProt Synonym Gene Names: GALT; GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
• UniProt Entry Name: B4GT1_BOVIN

Uniprot Description

Function: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Catalytic activity: UDP-alpha-D-galactose + D-glucose = UDP + lactose.UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor: Manganese.

Pathway: Protein modification; protein glycosylation.

Subunit structure: Homodimer; and heterodimer with alpha-lactabulmin to form lactose synthase.

Subcellular location: Isoform Long: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Note: Found in trans cisternae of Golgi.Isoform Short: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Found in trans cisternae of Golgi.Processed beta-1,4-galactosyltransferase 1: Secreted. Note: Soluble form found in body fluids.

Post-translational modification: The soluble form derives from the membrane forms by proteolytic processing.

Sequence similarities: Belongs to the glycosyltransferase 7 family.