p300/CBP elisa kit

p300/CBP Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500442

• Gene Names: KAT2B; CAF; PCAF; P/CAF
• Reactivity: Human, Mouse, Rat
• Synonyms: p300/CBP; p300/CBP Colorimetric Cell-Based ELISA Kit; CREB-binding protein; CREBBP E1A-associated protein p300; EP300; P300 transcription coactivator homolog; p300/CBP elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 832

• Preparation and Storage: Store at 4 degree C.

Related Product Information for p300/CBP elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for p300/CBP elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 40805843
• NCBI GeneID: 8850
• NCBI Accession #: NP_003875.3
• NCBI GenBank Nucleotide #: NM_003884.4
• UniProt Accession #: Q92831; Q6NSK1
• Molecular Weight: 93013
• NCBI Official Full Name: histone acetyltransferase KAT2B
• NCBI Official Synonym Full Names: lysine acetyltransferase 2B
• NCBI Official Symbol: KAT2B
• NCBI Official Synonym Symbols: CAF; PCAF; P/CAF
• NCBI Protein Information: histone acetyltransferase KAT2B
• UniProt Protein Name: Histone acetyltransferase KAT2B
• UniProt Gene Name: KAT2B
• UniProt Synonym Gene Names: PCAF; Histone acetylase PCAF; P/CAF

NCBI Description

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

PCAF: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family.

Protein type: Acetyltransferase; EC 2.3.1.48; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: A band; actomyosin; Ada2/Gcn5/Ada3 transcription activator complex; I band; kinetochore; nucleoplasm; nucleus; PCAF complex; protein complex

Molecular Function: acetyltransferase activity; chromatin binding; cyclin-dependent protein serine/threonine kinase inhibitor activity; histone acetyltransferase activity; histone deacetylase binding; lysine N-acetyltransferase activity; protein binding; protein complex binding; protein kinase binding; transcription coactivator activity; transcription cofactor activity; transcription factor binding

Biological Process: cell cycle arrest; cellular response to insulin stimulus; chromatin remodeling; internal peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein serine/threonine kinase activity; Notch signaling pathway; peptidyl-lysine acetylation; positive regulation of gene expression, epigenetic; positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; protein amino acid acetylation; protein deubiquitination; regulation of megakaryocyte differentiation; regulation of protein ADP-ribosylation; rhythmic process; transcription initiation from RNA polymerase II promoter; viral process