Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SNCB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a small family of proteins that inhibit phospholipase D2 and may function in neuronal plasticity. The encoded protein is abundant in lesions of patients with Alzheimer disease. A mutation in this gene was found in individuals with dementia with Lewy bodies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Uniprot Description
SNCB: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May play a role in neuronal plasticity, is abundant in neurofibrillary lesions of Alzheimer's patients. Highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5q35.2
Cellular Component: inclusion body
Molecular Function: calcium ion binding; phospholipase inhibitor activity; transition metal ion binding
Disease: Dementia, Lewy Body